Regiospecific effect of 1-octanol on cis - trans isomerization of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12

Autor: J.A. Killian, H.J. Heipieper, Gerrit Eggink, F.A. de Wolf, P.J. van der Meer, S. Isken, J.A.M. de Bont, P. de Waard
Rok vydání: 2001
Předmět:
Zdroj: ResearcherID
Applied Microbiology and Biotechnology 57 (2001)
Applied Microbiology and Biotechnology, 57, 541-547
ISSN: 1432-0614
0175-7598
Popis: The solvent-tolerant bacterium Pseudomonas putida S12, which adapts its membrane lipids to the presence of toxic solvents by a cis to trans isomerization of unsaturated fatty acids, was used to study possible in vivo regiospecificity of the isomerase. Cells were supplemented with linoleic acid (C18:2delta9-cis,delta12-cis), a fatty acid that cannot be synthesized by this bacterium, but which was incorporated into membrane lipids up to an amount of 15% of total fatty acids. After addition of 1-octanol, which was used as an activator of the cis-trans isomerase, the linoleic acid was converted into the delta9-trans,delta12-cis isomer, while the delta9-cis,delta12-trans and delta9-trans,epsilon12-trans isomers were not synthesized. Thus, for the first time, regiospecific in vivo formation of novel, mixed cis/trans isomers of dienoic fatty acid chains was observed. The maximal conversion (27-36% of the chains) was obtained at 0.03-0.04% (v/v) octanol, after 2 h. The observed regiospecificity of the enzyme, which is located in the periplasmic space, could be due to penetration of the enzyme to a specific depth in the membrane as well as to specific molecular recognition of the substrate molecules.
Databáze: OpenAIRE
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