A Lateral Flow Assay for Quantitative Detection of Amplified HIV-1 RNA
Autor: | Elizabeth Molyneux, Veronica Leautaud, Rebecca Richards-Kortum, Brittany A. Rohrman |
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Rok vydání: | 2012 |
Předmět: |
Viral Diseases
Health Screening lcsh:Medicine HIV Infections 02 engineering and technology Global Health Biochemistry 01 natural sciences Hiv 1 rna Nucleic Acids lcsh:Science Multidisciplinary Viral Load 021001 nanoscience & nanotechnology NASBA Clinical Laboratory Sciences 3. Good health AIDS Infectious Diseases Medicine RNA Viral Public Health 0210 nano-technology Viral load Research Article Biotechnology Test Evaluation Biomedical Engineering Sexually Transmitted Diseases Bioengineering Biology Lateral flow test Diagnostic Medicine Humans DNA Primers Base Sequence Oligonucleotide lcsh:R 010401 analytical chemistry HIV RNA Virology 0104 chemical sciences Bionanotechnology HIV-1 Nucleic acid Hiv patients lcsh:Q |
Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 9, p e45611 (2012) |
ISSN: | 1932-6203 |
Popis: | Although the accessibility of HIV treatment in developing nations has increased dramatically over the past decade, viral load testing to monitor the response of patients receiving therapy is often unavailable. Existing viral load technologies are often too expensive or resource-intensive for poor settings, and there is no appropriate HIV viral load test currently available at the point-of-care in low resource settings. Here, we present a lateral flow assay that employs gold nanoparticle probes and gold enhancement solution to detect amplified HIV RNA quantitatively. Preliminary results show that, when coupled with nucleic acid sequence based amplification (NASBA), this assay can detect concentrations of HIV RNA that match the clinically relevant range of viral loads found in HIV patients. The lateral flow test is inexpensive, simple and rapid to perform, and requires few resources. Our results suggest that the lateral flow assay may be integrated with amplification and sample preparation technologies to serve as an HIV viral load test for low-resource settings. |
Databáze: | OpenAIRE |
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