Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts
| Autor: | Minghao Ye, Daniel Batlle, Sasan Salmi, Michael S. LaPointe, Farhad R. Danesh |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Time Factors type 1 diabetes Cyclin D Human skin Tritium Retinoblastoma Protein glomerular mesangial cells S Phase Cyclin D1 Internal medicine medicine Humans cell growth Diabetic Nephropathies Kinase activity Phosphorylation Fibroblast Cells Cultured Cyclin Skin hyperplastic growth phenotype biology integumentary system Retinoblastoma protein G1 Phase Blood Proteins Fibroblasts Endocrinology medicine.anatomical_structure Diabetes Mellitus Type 1 Nephrology Cancer research biology.protein nephropathy Thymidine |
| Zdroj: | Kidney International. 56(4):1282-1285 |
| ISSN: | 0085-2538 |
| DOI: | 10.1046/j.1523-1755.1999.00690.x |
| Popis: | Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts. Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy. |
| Databáze: | OpenAIRE |
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