Reduction in E-cadherin expression fosters migration of Xenopus laevis primordial germ cells
| Autor: | Marieelen Oelkers, Thilo Baronsky, Juliane Melchert, Andreas Janshoff, Aliaksandr Dzementsei, Tomas Pieler |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Biophysics Xenopus Embryonic Germ Cells Xenopus Proteins Biochemistry 03 medical and health sciences Xenopus laevis Cell Movement medicine Cell Adhesion Animals Germ biology Transition (genetics) Cadherin Endoderm Force spectroscopy Gene Expression Regulation Developmental Embryo Adhesion biology.organism_classification Cadherins Cell biology 030104 developmental biology medicine.anatomical_structure Gene Knockdown Techniques embryonic structures Single-Cell Analysis |
| Zdroj: | Integrative biology : quantitative biosciences from nano to macro. 8(3) |
| ISSN: | 1757-9708 |
| Popis: | The transition from passive to active migration of primordial germ cells in Xenopus embryos correlates with a reduction in overall adhesion to surrounding endodermal cells as well as with reduced E-cadherin expression. Single cell force spectroscopy, in which cells are brought into brief contact with a gold surface functionalized with E-cadherin constructs, allows for a quantitative estimate of functional E-cadherin molecules on the cell surface. The adhesion force between migratory PGCs and the cadherin-coated surface was almost identical to cells where E-cadherin was knocked down by morpholino oligonucleotides (180 pN). In contrast, non-migratory PGCs display significantly higher adhesion forces (270 pN) on E-cadherin functionalised surfaces. On the basis of these observations, we propose that migration of PGCs in Xenopus embryos is regulated via modulation of E-cadherin expression levels, allowing these cells to move more freely if the level of E-cadherin is reduced. |
| Databáze: | OpenAIRE |
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