Growth Hormone-releasing factor releases acth from an AtT-20 mouse pituitary tumor cell line but not from normal pituitary cells
| Autor: | Paula Kardos, Toschichi Nakane, Tapan Audhya, Charles S. Hollander, Jir S. Tsai, Cynthia Brown |
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| Rok vydání: | 1987 |
| Předmět: |
endocrine system
medicine.medical_specialty Pituitary gland Corticotropin-Releasing Hormone Radioimmunoassay Peptide hormone Biology Growth Hormone-Releasing Hormone Dexamethasone General Biochemistry Genetics and Molecular Biology Cell Line Mice Adrenocorticotropic Hormone Anterior pituitary Pituitary Gland Anterior Internal medicine Cyclic AMP medicine Animals Pituitary Neoplasms ACTH receptor General Pharmacology Toxicology and Pharmaceutics Receptor Cells Cultured Pituitary tumors General Medicine medicine.disease medicine.anatomical_structure Endocrinology Somatostatin Cell culture hormones hormone substitutes and hormone antagonists |
| Zdroj: | Life Sciences. 40:2161-2167 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(87)90006-3 |
| Popis: | Corticotropin-releasing factor (CRF) and both human pancreatic growth hormone-releasing factor (hp-GRF) and rat hypothalamic GRF (rh-GRF) stimulated ACTH release from neoplastic AtT-20 mouse pituitary tumor cells in a dose-dependent fashion, with CRF inducing a 10-fold increase and GRF a maximal increment of approximately one-half that of CRF. Neither rh-GRF nor hp-GRF induced ACTH release in normal anterior pituitary cells. Pretreatment with either dexamethasone or somatostatin prior to the addition of rh-GRF inhibited the increase in ACTH release. Both ovine CRF and rh-GRF stimulated adenosine 3,5-monophosphate production in AtT-20 cells. The weak but clearly discernible effect of GRF on ACTH release from AtT-20 cells may be due to an abnormality in the AtT-20 cell receptor. |
| Databáze: | OpenAIRE |
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