Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
| Autor: | Seung Uk Jeong, Kyoung Ah Kang, Jin Won Hyun, Kristina Shilnikova, Pattage Madushan Dilhara Jayatissa Fernando, Jeong Eon Park, Soo-Young Na, Cheng Wen Yao, Mei Jing Piao, Yea Seong Ryu, Sun-Jin Boo, Min Chang Oh |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Colorectal cancer 5-Fluorouracil ATG5 Biochemistry Flow cytometry law.invention 03 medical and health sciences chemistry.chemical_compound Confocal microscopy law Internal medicine Drug Discovery medicine Autophagy Pharmacology chemistry.chemical_classification Reactive oxygen species medicine.diagnostic_test Acridine orange medicine.disease Molecular biology Staining Colon cancer 030104 developmental biology chemistry Molecular Medicine Original Article SNUC5/5-FUR |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 1976-9148 |
| Popis: | We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5- FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells. |
| Databáze: | OpenAIRE |
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