Sequential cellular and humoral responses in the abomasal mucosa and blood of Romney sheep dosed with Trichostrongylus axei
| Autor: | R.S. Green, W.E. Jonas, B. Rabel, R.J. Shaw, C.L. Shirer, P.G.C. Douch, T.K. Gatehouse, A. Pfeffer, S.A. Bisset |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Cellular immunity Trichostrongylus Antibodies Helminth CD4-CD8 Ratio Immunoglobulins Sheep Diseases Biology Romney sheep Immunoglobulin E Andrology Leukocyte Count Immune system Intestine Small medicine Animals Mast Cells Intestinal Mucosa Parasite Egg Count Immunity Cellular Sheep Abomasum Trichostrongylosis Eosinophil Mast cell Lymphocyte Subsets Eosinophils Infectious Diseases medicine.anatomical_structure Gastric Mucosa Immunology Trichostrongylus axei biology.protein Parasitology Antibody |
| Zdroj: | International journal for parasitology. 26(7) |
| ISSN: | 0020-7519 |
| Popis: | Sequential cellular and humoral responses in the abomasal mucosa and blood of Romney sheep dosed with Trichostrongylus axei. International Journal for Parasitelogy 26: 765–773. Abomasal cannalae were surgically placed in 7 2-year-old New Zealand Romney sheep which had been maintained parasite-free from birth. Four of these sheep were randomly selected and dosed orally with 10,000 infective Trichostrongylus axei larvae per week for 8 weeks, while the remsining 3 sheep served as unfufected controls. Abomasal biopsy, blood and faecal samples were obtained from all sheep at regular intervain from 5 days before and until 58 days after the first infection. The sheep were then killed, worm burdens assessed and abomasal and small intestinal samples collected. Faccal egg counts of all 4 dosed sheep were low and only one (No. 701) had a substantial worm burden (8400) post mortem. Overall, levels of mucosal mast cells/globule leukocytes, eosinophils, T19+ cells and larval migration inhibitory activity incressed signlfleastly in the abomasal mucosa of the dosed sheep compared to the controls. The CD4+:CD8+ cell ratio in the abornasal mucosa of the dosed sheep also increased compared to that of the controls (P=0.06). In blod, T. axei-specific antibody (total and IgG1) and eosinophil numbers increased signiflenatly in the dosed sheep. Mucosal cells staining for IgE (IgE+), and blood and mucosal eosinophils showed the earliest substantive increases in number followed by increases in specific serum antibody levels, numbers of mucosal cells fluorescing under UV light (UVf) and T19+ cells. The difference in the IgE+ and UVf cell response indicated that expession of globule leukocyte numbers lagged behind that of mucosal mast cells. The results supported the concept of CD4+ T cell help in the abomasal mucosa and defined the sequential expression of compoments of the immunological responses potentially mediating resistance to T. axei. In sheep No. 701, persistence of adult worms was associated with lower mucosal IgE+ cell and eosinophil responses compared with the other dosed sheep. |
| Databáze: | OpenAIRE |
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