Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes
Autor: | Swum Yi Kyu, Ki-Byung Lim, Kyeung Il Park, Chang Kil Kim, Je Min Lee, Phyo Phyo Win Pe, Aung Htay Naing |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Phytoene desaturase Carotenoid biosynthesis Plant Science lcsh:Plant culture 01 natural sciences 03 medical and health sciences Lipoxygenase VIGS Pepper Genetics Gene silencing lcsh:SB1-1110 lcsh:QH301-705.5 Gene Oxidase test biology Research fungi food and beverages Ethylene biosynthesis Ripening Fruit ripening Phenotype Cell biology Relative gene expression 030104 developmental biology lcsh:Biology (General) biology.protein 010606 plant biology & botany Biotechnology |
Zdroj: | Plant Methods, Vol 15, Iss 1, Pp 1-10 (2019) Plant Methods |
ISSN: | 1746-4811 |
DOI: | 10.1186/s13007-019-0491-z |
Popis: | Background Past research has shown that virus-induced phytoene desaturase (PDS) gene silencing via agroinjection in the attached and detached fruit of tomato plants results in a pale-yellow fruit phenotype. Although the PDS gene is often used as a marker for gene silencing in tomatoes, little is known about the role of PDS in fruit ripening. In this study, we investigated whether the pepper PDS gene silenced endogenous PDS genes in the fruit of two tomato cultivars, Dotaerang Plus and Legend Summer. Results We found that the pepper PDS gene successfully silenced endogenous PDS in tomato fruit at a silencing frequency of 100% for both cultivars. A pale-yellow silenced area was observed over virtually the entire surface of individual fruit due to the transcriptional reduction in phytoene desaturase (PDS), zeta-carotene (ZDS), prolycopene isomerase (CrtlSO), and beta-carotene hydroxylase (CrtR-b2), which are the carotenoid biosynthesis genes responsible for the red coloration in tomatoes. PDS silencing also affected the expression levels of the fruit-ripening genes Tomato AGAMOUS-LIKE1 (TAGL1), RIPENING INHIBITOR (RIN), pectin esterase gene (PE), lipoxygenase (LOX), FRUITFULL1/FRUITFUL2 (FUL1/FUL2), and the ethylene biosynthesis and response genes 1-aminocyclopropane-1-carboxylate oxidase 1 and 3 (ACO1 and ACO3) and ethylene-responsive genes (E4 and E8). Conclusion These results suggest that PDS is a positive regulator of ripening in tomato fruit, which must be considered when using it as a marker for virus-induced gene silencing (VIGS) experiments in order to avoid fruit-ripening side effects. |
Databáze: | OpenAIRE |
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