Profiling the Secretion of Soluble Mediators by End Stage Osteoarthritis Synovial Tissue Explants Reveals a Reduced Responsiveness to an Inflammatory Trigger
| Autor: | Gerjo J.V.M. van Osch, Benno van El, Anne-Marie Zuurmond, Reinout Stoop, Frits van der Ham, Jan H. Verheijen, Margreet Kloppenburg, A. Koudijs, Vedrana Stojanovic-Susulic, Tom W J Huizinga, Lobke Gierman |
|---|---|
| Přispěvatelé: | Pathology, Otorhinolaryngology and Head and Neck Surgery |
| Rok vydání: | 2013 |
| Předmět: |
Cartilage
Articular Male Anatomy and Physiology Glycobiology Biomedical Innovation lcsh:Medicine Osteoarthritis Matrix metalloproteinase Biochemistry Tissue Culture Techniques Glycosaminoglycan Life Interleukin-1alpha lcsh:Science Musculoskeletal System Glycosaminoglycans Multidisciplinary Chemistry Middle Aged Bovine Cartilage medicine.anatomical_structure Medicine Cytokines Proteoglycans Inflammation Mediators EELS - Earth Environmental and Life Sciences medicine.symptom MHR - Metabolic Health Research Healthy Living Research Article medicine.medical_specialty Histology Adolescent Inflammation Rheumatology Internal medicine medicine Animals Humans Secretion Biology Aged Cartilage lcsh:R Immunity medicine.disease Matrix Metalloproteinases Endocrinology Immune System Immunology Immunologic Techniques Clinical Immunology Cattle lcsh:Q Joint Capsule Explant culture |
| Zdroj: | PLoS ONE, Vol 8, Iss 5, p e62634 (2013) PLoS ONE, 8(5) PLoS ONE, 5, 8 PLoS ONE PLoS One (print), 8(5). Public Library of Science |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0062634 |
| Popis: | Objective:Evidence is accumulating that synovial tissue plays an active role in osteoarthritis (OA), however, exact understanding of its contribution is lacking. In order to further elucidate its role in the OA process, we aimed to identify the secretion pattern of soluble mediators by synovial tissue and to assess its ability to initiate cartilage degeneration.Methods:Synovial tissue explants (STEs) obtained from donors without history of OA (n = 8) or from end stage OA patients (n = 16) were cultured alone or together with bovine cartilage explants in the absence or presence of IL-1α. The secretion of 48 soluble mediators was measured and the effect on glycosaminoglycan (GAG) release and matrix metalloproteinase (MMP) activity was determined.Results:Normal and OA STEs secreted comparable levels of almost all measured soluble mediators. However, in the presence of IL-1α these mediators were less secreted by OA than by normal STEs of which 15 differed significantly (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|