Arterial Network Geometric Characteristics and Regulation of Capillary Blood Flow in Hamster Skeletal Muscle Microcirculation
| Autor: | Mauro Ursino, Antonio Colantuoni, Noemy Starita, Teresa Mastantuono, Martina Di Maro, Dominga Lapi |
|---|---|
| Přispěvatelé: | Lapi D., Di Maro M., Mastantuono T., Starita N., Ursino M., Colantuoni A. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
arteriolar rhythmic diameter changes Physiology blood flow regulation Hamster L-arginine Vasomotion 030204 cardiovascular system & hematology lcsh:Physiology Arteriolar rhythmic diameter change Constriction Microcirculation 03 medical and health sciences 0302 clinical medicine capillary perfusion Physiology (medical) medicine Strahler's ordering scheme skeletal muscle microcirculation Original Research Papaverine lcsh:QP1-981 papaverine Chemistry Skeletal muscle Blood flow Anatomy 030104 developmental biology medicine.anatomical_structure Perfusion medicine.drug |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 9 (2019) |
| ISSN: | 1664-042X |
| Popis: | This study was aimed to characterize the geometric arrangement of hamster skeletal muscle arteriolar networks and to assess the in vivo rhythmic diameter changes of arterioles to clarify regulatory mechanisms of the capillary perfusion. The experimental study was carried out in male Syrian hamsters implanted with a plastic chamber in the dorsum skin under pentobarbital anesthesia. The skeletal muscle microvessels were visualized by fluorescence microscopy. The vessel diameters, lengths and the rhythmic diameter changes of arterioles were analyzed with computer-assisted techniques. The arterioles were classified according to a centripetal ordering scheme. In hamster skeletal muscle microvasculature the terminal branchings, differentiated in long and short terminal arteriolar trees (TATs), originated from anastomotic vessels, defined "arcading" arterioles. The long TATs presented different frequencies along the branching vessels; order 4 arterioles had frequencies lower than those observed in the order 3, 2, and 1 vessels. The short TAT order 3 arterioles, directly originating from "arcading" parent vessels, showed a frequency dominating all daughter arterioles. The amplitude of diameter variations in larger vessels was in the range 30-40% of mean diameter, while it was 80-100% in order 3, 2, and 1 vessels. Therefore, the complete constriction of arterioles, caused an intermittent capillary blood perfusion. L-arginine or papaverine infusion caused dilation of arterioles and transient disappearing of vasomotion waves and induced perfusion of all capillaries spreading from short and long TAT arrangements. Therefore, the capillary blood flow was modulated by changes in diameter of terminal arterioles penetrating within the skeletal muscle fibers, facilitating redistribution of blood flow according to the metabolic demands of tissues. |
| Databáze: | OpenAIRE |
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