Suscetibilidade da linhagem de células Vero a cepas vacinais do vírus do sarampo
| Autor: | Inácio França Mendes, Flávia Saldanha Kubrusly, Célia Sayoko Takata, Cosue Miyaki, Edda de Rizzo |
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| Jazyk: | portugalština |
| Rok vydání: | 1994 |
| Předmět: |
Vírus do sarampo
Strain (chemistry) lcsh:Public aspects of medicine Vírus do sarampo/imunologia Public Health Environmental and Occupational Health Suscetibilidade celular lcsh:RA1-1270 Biology biology.organism_classification Virology Cell susceptibility Measles virus Vaccine Potency measles vaccine Viral replication Cell culture measles virus Vacina contra sarampo/normas Vero cell Potency cell susceptibility Measles vaccine Vacina contra sarampo |
| Zdroj: | Revista de Saúde Pública v.28 n.3 1994 Revista de Saúde Pública Universidade de São Paulo (USP) instacron:USP Revista de Saúde Pública, Volume: 28, Issue: 3, Pages: 209-212, Published: JUN 1994 Revista de Saúde Pública, Vol 28, Iss 3, Pp 209-212 (1994) Revista de Saúde Pública, Vol 28, Iss 3, Pp 209-212 |
| Popis: | A suscetibilidade da linhagem de células Vero ao vírus do sarampo é bem conhecida e sua utilização no controle da potência da vacina contra o sarampo é amplamente difundida. Com o objetivo de comparar a suscetibilidade de células Vero empregadas em titulações, amostras provenientes de dois laboratórios controladores (Vero IB e Vero INCQS), foram testadas frente a três cepas vacinais: Moraten, Schwarz e Biken CAM-70. Foram titulados 72 lotes de vacinas contra o sarampo, sendo 25 produzidos com a cepa Moraten, 24 com a cepa Schwarz e 23 com a cepa Biken CAM-70. A análise estatística dos resultados obtidos nas titulações, feita através dos testes Limites para uma Média e "t" de Student, mostrou que para as cepas Moraten e Biken CAM-70, as diferenças de títulos não foram estatisticamente significantes, o mesmo não ocorrendo com a cepa Schwarz, para a qual as células Vero IB se mostraram mais sensíveis. Vero cells used by distinct measles vaccine control laboratories had their susceptibility to Moraten, Schwarz and Biken CAM-70 vaccine strains assayed. Of a total of 72 lots of measles vaccine whose potency was titrated by microtechnique in two Vero cell samples (Vero IB and Vero INCQS), 25 had been produced with Moraten strain, 24 with Schwarz and 23 with Biken CAM-70. The statistical analysis of the results demonstrated that both Vero cells assayed presented comparable susceptibility to Moraten and Biken CAM-70 strains. As to the Schwarz strain, Vero IB cells were more susceptible than the other cell sample tested, thus confirming the existence of different sensitivities of Vero cells to some measles vaccine strains, or even to viruses derived from the same strain but with different passage histories. An altered cell susceptibility to virus replication may significantly alter the results in potency testing. Such alteration may be caused not only by the adoption of distinct protocols for the maintenance of cell cultures by different control laboratories but also by the methodology followed in the vaccine titration. In order to minimize the differences existing among the results obtained in the potency testing, it is suggested that all control laboratories should use the same protocols for cell culture maintenance as well as for vaccine potency testing. |
| Databáze: | OpenAIRE |
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