Multilocus differentiation of the related dermatophytes Microsporum canis, Microsporum ferrugineum and Microsporum audouinii
| Autor: | Mohammad Javad Najafzadeh, Kazuo Satoh, Mohammad Reza Shidfar, Koichi Makimura, G. Sybren de Hoog, Hossein Mirhendi, Ali Rezaei-Matehkolaei |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Microsporum audouinii Molecular Sequence Data Iran Biology Microbiology Peptide Elongation Factor 1 Species Specificity Tinea Tubulin Translation elongation DNA Ribosomal Spacer Prevalence Animals Dermatomycoses Humans Microsporum Species identification Amino Acid Sequence Microsporum canis Internal transcribed spacer Mycological Typing Techniques Its region Sequence Analysis DNA General Medicine biology.organism_classification Restriction enzyme Phenotype Microsporum ferrugineum Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Medical Microbiology, 61(1), 57-63. Society for General Microbiology |
| ISSN: | 0022-2615 |
| Popis: | Microsporum ferrugineum, an uncommon causative agent of dermatophytosis, has restricted endemicity. Iranian strains suspected to be M. ferrugineum from two patients with tinea were analysed using the rDNA internal transcribed spacer (ITS) region and the partial beta-tubulin (BT2) and translation elongation factor 1-alpha (TEF1) genes. Strains were compared to reference strains to differentiate M. ferrugineum from its relatives Microsporum canis and Microsporum audouinii. Inter-species differences for TEF1 and BT2 were found to be higher than for the ITS region, which is the current molecular standard for species identification in dermatophytes. Intra-species variation was zero for each of the markers. In silico analysis showed that the restriction enzymes Banl and BshNI were together sufficient to differentiate the three species based on TEF1, whereas a two-step digestion was needed with BT2 or the ITS region. The prevalence of M. ferrugineum in clinical samples in Iran appeared to be higher than suspected on the basis of routine phenotypic identification. |
| Databáze: | OpenAIRE |
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