Cloning and identification of porcine programmed death 1
| Autor: | Guangzhi Tong, Jin-Mei Peng, Yong-Qian Yang, Yao Wang, Tong-Qing An, Zhi-Jun Tian, Deng-Yun Li, Jiazeng Chen, Yan-Jun Zhou, Heng-Gui Liu |
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| Rok vydání: | 2010 |
| Předmět: |
Sequence analysis
T-Lymphocytes T cell Molecular Sequence Data Programmed Cell Death 1 Receptor Sus scrofa Immunology Immunoglobulins In Vitro Techniques Lymphocyte Activation Conserved sequence Mice Immune system Antigen Antigens CD medicine Animals Humans Amino Acid Sequence Cloning Molecular Peptide sequence Conserved Sequence Cell Proliferation DNA Primers Cloning Base Sequence Sequence Homology Amino Acid General Veterinary biology Molecular biology medicine.anatomical_structure Antigens Surface biology.protein Antibody Apoptosis Regulatory Proteins |
| Zdroj: | Veterinary Immunology and Immunopathology. 136:157-162 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2010.03.001 |
| Popis: | Programmed death 1 (PD-1) is a member of the immunoglobulin (Ig) superfamily, which is expressed on activated T cells, B cells and monocytes. Many researches have demonstrated that a high level of PD-1 expression is closely related to persistent infection and immune evasion in some human infections. In order to study the relationship between PD-1 expression and persistent infections caused by some porcine viruses, we first cloned the porcine PD-1 from porcine PBMCs based on the blast result in the EST database using the human PD-1 sequence. Sequence analysis showed that the cloned PD-1 molecule shares 63 and 54% amino acid sequence identity with human and murine PD-1, respectively. Its molecular structure is also similar to that of human and murine PD-1, containing an IgV-like domain in the extracellular region and two immune regulatory motifs in its cytoplasmic tail. The in vitro T cell proliferation assay showed that the cloned PD-1 could inhibit porcine T cell proliferation by 71% and secretion of IFN-gamma and IL-2 by 64 and 53%, respectively. These data suggest that porcine PD-1 negatively regulates the porcine immune response in a similar manner to that of its counterpart in the human and mouse immune system. |
| Databáze: | OpenAIRE |
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