Pharmacology and molecular docking study of cartilage protection of Chinese herbal medicine Fufang Shatai Heji (STHJ) by inhibiting the expression of MMPs in collagen-induced arthritis mice
| Autor: | Si-Jia Chen, Yun-Wu Li, Qi-Shan Wang, Jing Wu, Bing-Xin Xu, Kai-Jian Fan, Tingyu Wang, Hui Teng |
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| Rok vydání: | 2022 |
| Předmět: |
Advanced and Specialized Nursing
business.industry Cartilage ADAMTS Arthritis Matrix metalloproteinase Pharmacology medicine.disease Arthritis Experimental Matrix Metalloproteinases Molecular Docking Simulation Mice Anesthesiology and Pain Medicine Immune system medicine.anatomical_structure Synovitis Rheumatoid arthritis medicine Animals Immunohistochemistry business Drugs Chinese Herbal |
| Zdroj: | Annals of Palliative Medicine. 11:466-479 |
| ISSN: | 2224-5839 2224-5820 |
| Popis: | Background This study aims to explore whether Fufang Shatai Heji (STHJ), as a mixture collected by a decoction of a variety of Chinese herbal medicines for immune system diseases, can improve the cartilage destruction of rheumatoid arthritis (RA). Methods The therapeutic effects of STHJ were studied using collagen induced arthritis (CIA) mice. The improvement effect of STHJ on synovitis and cartilage damage caused by arthritis was studied by joint pathological analysis. The inhibitory effect of STHJ on related degradation enzymes in cartilage was studied by immunohistochemistry and real-time polymerase chain reaction (PCR). The specific targets of STHJ were predicted by molecular docking. Results After successfully inducing CIA, the paws of the mice showed significant swelling, and athological analysis of the ankle and knee joints also showed significant cartilage destruction and synovial hyperplasia. However, synovial hyperplasia and cartilage destruction were markedly alleviated after administration of STHJ. And after SHTJ treatment, the expression of ADAMTS-4, ADAMTS-5, MMP-9 and MMP-13, in the cartilage layer of CIA mice was significantly inhibited. Through molecular docking assays, we proved that acteoside in STHJ could directly bind to the Glu111, Phe110 residues in MMP-9 and glycyrrhizic acid in STHJ bind to the Glu382, Asn433 residues in MMP-13. Conclusions Our results suggested that STHJ may alleviate synovial hyperplasia and cartilage destruction in CIA mice and protect cartilage by inhibiting the expression of MMP-9 and other enzymes. |
| Databáze: | OpenAIRE |
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