CRISPR-Cas technology in corn: a new key to unlock genetic knowledge and create novel products
| Autor: | Ken S. Heyndrickx, Hannes Claeys, Michael L. Nuccio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Genomic data Plant Science Biology 01 natural sciences Data science Article 03 medical and health sciences 030104 developmental biology Genetics Key (cryptography) CRISPR Identification (biology) Target gene Agronomy and Crop Science Molecular Biology 010606 plant biology & botany Biotechnology |
| Zdroj: | Mol Breed |
| Popis: | Since its inception in 2012, CRISPR-Cas technologies have taken the life science community by storm. Maize genetics research is no exception. Investigators around the world have adapted CRISPR tools to advance maize genetics research in many ways. The principle application has been targeted mutagenesis to confirm candidate genes identified using map-based methods. Researchers are also developing tools to more effectively apply CRISPR-Cas technologies to maize because successful application of CRISPR-Cas relies on target gene identification, guide RNA development, vector design and construction, CRISPR-Cas reagent delivery to maize tissues, and plant characterization, each contributing unique challenges to CRISPR-Cas efficacy. Recent advances continue to chip away at major barriers that prevent more widespread use of CRISPR-Cas technologies in maize, including germplasm-independent delivery of CRISPR-Cas reagents and production of high-resolution genomic data in relevant germplasm to facilitate CRISPR-Cas experimental design. This has led to the development of novel breeding tools to advance maize genetics and demonstrations of how CRISPR-Cas technologies might be used to enhance maize germplasm. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-021-01200-9. |
| Databáze: | OpenAIRE |
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