Inhibitory effects of fucoidan on NMDA receptors and l-type Ca2+ channels regulating the Ca2+ responses in rat neurons

Autor: Shuibo Gao, Susumu Terakawa, Hong Wu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
N-Methylaspartate
Calcium Channels
L-Type
Pharmaceutical Science
Fucus vesiculosus
Glutamic Acid
RM1-950
Inhibitory postsynaptic potential
mRNA expression of PR1/PR2
Hippocampus
Receptors
N-Methyl-D-Aspartate
chemistry.chemical_compound
Cerebellar Cortex
Polysaccharides
Drug Discovery
Excitatory Amino Acid Agonists
Animals
Receptors
AMPA
Rats
Wistar
Cells
Cultured
Pharmacology
Neurons
biology
Fucoidan
Ca2+ transient
L type ca2 channels
General Medicine
3-Pyridinecarboxylic acid
1
4-dihydro-2
6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-
Methyl ester
biology.organism_classification
Fucaceae
Rats
Brown algae
Complementary and alternative medicine
chemistry
Biochemistry
nervous system
Receptors
Glutamate
Sulphated polysaccharides
Molecular Medicine
NMDA receptor
Calcium
Therapeutics. Pharmacology
Cortical and hippocampal neuron
sulphated polysaccharide
Research Article
Zdroj: Pharmaceutical Biology
Pharmaceutical Biology, Vol 57, Iss 1, Pp 1-7 (2019)
ISSN: 1744-5116
1388-0209
Popis: Context: Fucoidan, a sulphated polysaccharide extracted from brown algae [Fucus vesiculosus Linn. (Fucaceae)], has multiple biological activities. Objective: The effects of fucoidan on Ca2+ responses of rat neurons and its probable mechanisms with focus on glutamate receptors were examined. Materials and methods: The neurons isolated from the cortex and hippocampi of Wistar rats in postnatal day 1 were employed. The intracellular Ca2+ responses triggered by various stimuli were measured in vitro by Fura-2/AM. Fucoidan at 0.5 mg/mL or 1.5 mg/mL was applied for 3 min to determine its effects on Ca2+ responses. RT-PCR was used to determine the mRNA expression of neuron receptors treated with fucoidan at 0.5 mg/mL for 3 h. Results: The Ca2+ responses induced by NMDA were 100% suppressed by fucoidan, and those induced by Bay K8644 90% in the cortical neurons. However, fucoidan has no significant effect on the Ca2+ responses of cortical neurons induced by AMPA or quisqualate. Meanwhile, the Ca2+ responses of hippocampal neurons induced by glutamate, ACPD or adrenaline, showed only a slight decrease following fucoidan treatment. RT-PCR assays of cortical and hippocampal neurons showed that fucoidan treatment significantly decreased the mRNA expression of NMDA-NR1 receptor and the primer pair for l-type Ca2+ channels, PR1/PR2. Discussion and conclusions: Our data indicate that fucoidan suppresses the intracellular Ca2+ responses by selectively inhibiting NMDA receptors in cortical neurons and l-type Ca2+ channels in hippocampal neurons. A wide spectrum of fucoidan binding to cell membrane may be useful for designing a general purpose drug in future.
Databáze: OpenAIRE
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