A novel transcription factor negatively regulates antioxidant response, cell wall integrity and virulence in the fungal insect pathogen, Beauveria bassiana
| Autor: | Xin Zhao, Zhibing Luo, Ning Peng, Yongjun Zhang, Tingying Luo, Ying Yin, Shuaishuai Huang |
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| Rok vydání: | 2021 |
| Předmět: |
Leucine zipper
Insecta Virulence Biology Microbiology Antioxidants Superoxide dismutase Fungal Proteins 03 medical and health sciences Transcription (biology) Cell Wall Gene expression Animals Beauveria Transcription factor Pathogen Gene Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences 030306 microbiology Spores Fungal Cell biology biology.protein Transcription Factors |
| Zdroj: | Environmental microbiologyReferences. 23(9) |
| ISSN: | 1462-2920 |
| Popis: | Genomic data have identified a class of fungal specific transcription factors (FsTFs) that are thought to regulate unique aspects of fungal gene expression, although the functions of many of these proteins remain unknown. Here, a novel FsTF (BbStf1), which features a leucine zipper dimerization domain and a fungal transcription factor regulatory middle homology region, was characterized in Beauveria bassiana, a filamentous insect fungal pathogen. Transcriptional activation and nuclear localization were experimentally confirmed for BbStf1. Disruption of Bbstf1 resulted in increased tolerance to oxidative stress and cell wall perturbation, accompanied by increased peroxidase (POD) and superoxide dismutase (SOD) activities and ratio of reduced/oxidized glutathione (GSH/GSSG), and by thickened cell wall and altered composition. Gene expression profile analysis revealed that transcription patterns of antioxidant enzyme and cell wall integrity-involved genes were altered in the ∆Bbstf1, including some BbStf1-targeted genes clarified with evidence. The ∆Bbstf1 strain displayed greater virulence to Galleria mellonella in the bioassays through both topical infection and intrahaemocoel injection due to more rapid proliferation in the haemocoel as compared to the wild-type strain. Altogether, BbStf1 acts as a negative regulator of antioxidant response, cell wall integrity and virulence in B. bassiana. |
| Databáze: | OpenAIRE |
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