Evaluation of amygdalin-loaded alginate-chitosan nanoparticles as biocompatible drug delivery carriers for anticancerous efficacy
| Autor: | Shah Rukh Abbas, Rabia Sohail |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lung Neoplasms
Amygdalin Dispersity Nanoparticle Antineoplastic Agents 02 engineering and technology Biochemistry 03 medical and health sciences chemistry.chemical_compound Structural Biology Cell Line Tumor Materials Testing medicine Mucoadhesion Humans Cytotoxicity Molecular Biology 030304 developmental biology Chitosan Drug Carriers 0303 health sciences Cationic polymerization General Medicine 021001 nanoscience & nanotechnology chemistry Drug delivery Biophysics Nanoparticles Drug Screening Assays Antitumor Swelling medicine.symptom 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 153:36-45 |
| ISSN: | 0141-8130 |
| Popis: | Amygdalin, despite possessing anticancerous properties, has been viewed as a controversial choice due to the presence of the cyanide group. Here, we synthesise and investigate the potential of alginate–chitosan nanoparticles (ACNPs) as drug delivery agents for amygdalin encapsulation and its delivery to cancer cells. Amygdalin loaded ACNPs were made with both anionic and cationic outer layer to further investigate charge dependency on drug delivery and cytotoxicity. ACNPs encapsulating amygdalin were monodisperse, colloidally stable with ~90% drug encapsulation efficiency and were entirely made from natural materials. The nanoparticles exhibited sustained drug release for a duration of 10 h and significant swelling rates in neutral and slightly acidic environments. The ACNPs successfully adhered to porcine mucin type II when assessed for its mucoadhesion and shown to transmigrate with an average velocity of 1.68 μm/s in uncoated channels, under biomimicked flow conditions. To investigate charge dependency on drug delivery and cytotoxicity, amygdalin loaded ACNPs were made with both anionic and cationic outer layer and assessed. ACNPs demonstrated greater yet sustained anti-cancerous effect on H1299 cell lines in a dose-dependent manner than free amygdalin suggesting greater cellular uptake of the former. In conclusion, biocompatible and biodegradable alginate–chitosan nanoparticles can be used as an effective drug delivery system for sustained and controlled amygdalin release with its improved cytotoxic effect on cancerous cells while protecting normal cells and tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect