Calcium sensing receptor effects in adipocytes and liver cells: Implications for an adipose-hepatic crosstalk
| Autor: | Andrea Arreguin, Pia Villarroel, Marco Arrese, Mariana Cifuentes, Cecilia Fuentes, Pamela Mattar, Amanda D'Espessailles, Marcela Reyes |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cinacalcet Cell Survival Biophysics Adipose tissue Biology Fatty Acid-Binding Proteins Biochemistry Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Adipocyte medicine Adipocytes Humans Molecular Biology Triglycerides Inflammation Adipogenesis Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Hep G2 Cells medicine.disease 030104 developmental biology Endocrinology chemistry Adipose Tissue Liver 030220 oncology & carcinogenesis Lipogenesis Hepatic stellate cell Cytokines RNA Steatosis Calcium-sensing receptor Receptors Calcium-Sensing medicine.drug |
| Zdroj: | Archives Of Biochemistry And Biophysics Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1096-0384 |
| Popis: | The calcium sensing receptor (CaSR) is expressed in human adipose cells, and its activation may associate with adipose tissue (AT) dysfunction. We evaluated whether CaSR stimulation influences adipocyte triglyceride (TG) and fatty acid binding protein 4 (aP2) content, and hepatocyte TGs and proinflammatory cytokine expression. The effect of the calcimimetic cinacalcet on TGs (fluorimetry), lipogenic genes (qPCR) and aP2 (immunoblot) was evaluated in LS14 adipocytes or AT. In the human HepG2 hepatic cell line, we assessed CaSR expression and cinacalcet effect on TGs and lipogenic and proinflammatory genes. CaSR activation decreased adipocyte TG content by 20% and the expression of GPD and LPL by 34% and 20%, respectively. Cinacalcet increased aP2 protein expression by 60%. CaSR expression was shown in HepG2 cells and human liver samples. Cinacalcet-treated HepG2 cells in the presence of oleic acid exhibited a19% increased TG content. No changes were observed in the expression of lipogenic genes in HepG2 cells, however there was a 50%-300% elevation in the expression of proinflammatory cytokines. CaSR activation in adipocytes may associate with decreased TG storage ability and increased aP2. Hepatic CaSR stimulation may elevate steatosis and proinflammatory factors. We propose that CaSR may contribute to obesity-associated hepatic metabolic consequences. |
| Databáze: | OpenAIRE |
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