Estradiol positive feedback on the rat anterior pituitary gland in vitro
| Autor: | Wan-Song A. Wun, Ian H. Thorneycroft |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Pituitary gland
medicine.medical_specialty Gonadotropin RH Stimulation In Vitro Techniques Peptide hormone Biochemistry Feedback Gonadotropin-Releasing Hormone Endocrinology Anterior pituitary Pituitary Gland Anterior Internal medicine medicine Animals Molecular Biology Positive feedback Estradiol Chemistry Luteinizing Hormone In vitro Rats medicine.anatomical_structure Female Follicle Stimulating Hormone |
| Zdroj: | Molecular and Cellular Endocrinology. 54:165-169 |
| ISSN: | 0303-7207 |
| Popis: | LH surges can be elicited by estradiol (E 2 ). The mechanism could be either by induction of a surge of hypothalamic LH-RH or by directly increasing the responsiveness of the pituitary gland to hypothalamic LH-RH without an LH-RH surge. In order to study the positive feedback of E 2 on the anterior pituitary gland, without any possible influence of an LH-RH surge, we utilized long-term in vitro superfusion of the anterior pituitary gland. The E 2 concentration was 100 pg/ml and 10 -8 M LH-RH was pulsed every 30 min for 3 min. LH and FSH surge was detected about 21 h after the beginning of the E 2 infusion in all three repeated experiments. No surge was detected in any other channels, i.e. control, E 2 superfusion alone or LH-RH stimulation alone. This study demonstrates that in the rat the induction of the preovulatory LH peak can be accomplished by a direct effect of E 2 upon the anterior pituitary gland, provided it is being stimulated by constant pulses of LH-RH. |
| Databáze: | OpenAIRE |
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