A novel, simplified and stability-indicating high-throughput ultra-fast liquid chromatography method for the determination of rosmarinic acid in nanoemulsions, porcine skin and nasal mucosa
Autor: | Bruna Medeiros-Neves, Kleyton Santos Veras, Marina Cardoso Nemitz, Valquiria Linck Bassani, Amélia T. Henriques, Letícia Scherer Koester, Helder Ferreira Teixeira, Flávia Nathiely Silveira Fachel |
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Rok vydání: | 2017 |
Předmět: |
Bioanalysis
Antioxidant Calibration curve Swine medicine.medical_treatment Clinical Biochemistry Mucous membrane of nose 02 engineering and technology 01 natural sciences Biochemistry Depsides Analytical Chemistry chemistry.chemical_compound Emulsification procedure Drug Stability Limit of Detection medicine Animals Skin Chromatography Rosmarinic acid 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Permeation 021001 nanoscience & nanotechnology 0104 chemical sciences High-Throughput Screening Assays Nanostructures Nasal Mucosa chemistry Cinnamates Linear Models Emulsions Particle size 0210 nano-technology Chromatography Liquid |
Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1083 |
ISSN: | 1873-376X |
Popis: | Currently, there is an increasing interest on the development of topical formulations containing rosmarinic acid (RA) due to its well-documented antioxidant activity. This study aimed to develop and validate a stability-indicating ultra-fast liquid chromatography (UFLC) method for the determination of RA in nanoemulsions, porcine skin and nasal mucosa intended to be applied in permeation/retention studies and for development of topical nanoemulsions. Chromatographic separation was carried out using a C18 column packed with 2.6 μm particle size in isocratic conditions using as mobile phase water:acetonitrile (83:17, v/v), acidified with 0.1% trifluoracetic acid (v/v), with a total time of analysis of 3.5 min and detection at 330 nm. RA analysis was specific in the presence of both non-biological (blank nanoemulsion and receptor fluid) and biological matrices (porcine ear skin and porcine nasal mucosa). No interference of degradation products of RA was verified after different stress conditions such as acidic, alkaline, oxidative, light exposure (UV-A and UV-C) and thermal demonstrating the method stability-indicating property. The analytical (0.1–10.0 μg·mL−1) and bioanalytical (0.5–10.0 μg·mL−1) linearity was proved by analysis of the calibration curves of RA and no matrix effect was observed. The method was sensitive, precise and accurate, and showed recovery higher than 85%. The method was considered robust as evaluated by a Plackett-Burman experimental design. In the validated conditions, the RA was determined in the nanoemulsions obtained by spontaneous emulsification procedure (1.007 ± 0.040 mg·mL−1), porcine ear skin (1.13 ± 0.19 μg·cm−2) and nasal mucosa (22.46 ± 3.99 μg·cm−2) after retention/permeation studies. Thus, a highly sensitive, simple, fast and stability-indicating method was developed for RA analysis during the development of topical nanoemulsions and bioanalytical assays in complex matrices. |
Databáze: | OpenAIRE |
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