Fluoroquinolone-resistant Brucella melitensis mutants obtained in vitro
| Autor: | R. Elisa Rodríguez-Tarazona, Juan Luis Muñoz-Bellido, Francisco García Lázaro, José Angel García-Rodríguez |
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| Rok vydání: | 2009 |
| Předmět: |
DNA Topoisomerase IV
DNA Bacterial Microbiology (medical) Ofloxacin Biovar Moxifloxacin Mutant Levofloxacin Microbial Sensitivity Tests Microbiology Agar plate Ciprofloxacin Drug Resistance Bacterial Brucella melitensis medicine heterocyclic compounds Pharmacology (medical) Norfloxacin Antibacterial agent Aza Compounds biology Chemistry Sequence Analysis DNA General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Infectious Diseases DNA Gyrase Mutation Quinolines bacteria Fluoroquinolones medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 34:252-254 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2008.12.013 |
| Popis: | Three fluoroquinolone-resistant Brucella melitensis mutants were obtained after successive passages on norfloxacin-containing agar plates. The original strain was B. melitensis biovar 2 ATCC 23457. Mutants emerged at frequencies ranging between 1 x 10(-6) and 3 x 10(-8). The three mutants showed a gyrA Ala71--Ser mutation as well as insertion of Ala340. No mutations were observed in the parC and gyrB genes. Minimum inhibitory concentrations (MICs) increased by four- to eight-fold from the wild-type to the first-step mutant for norfloxacin, ciprofloxacin, levofloxacin and moxifloxacin. MICs of the second-step mutant increased significantly only for norfloxacin and those for the third-step mutant for norfloxacin and ciprofloxacin, suggesting the involvement of some efflux mechanism. |
| Databáze: | OpenAIRE |
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