Mushrooms as potential therapeutic agents in the treatment of cancer: Evaluation of anti-glioma effects of Coprinus comatus, Cantharellus cibarius, Lycoperdon perlatum and Lactarius deliciosus extracts
| Autor: | Renata Markiewicz-Żukowska, Katarzyna Socha, Patryk Nowakowski, Krystyna Gromkowska-Kępka, Monika Grabia, Joanna Bielecka, Justyna Moskwa, Sylwia K. Naliwajko, Maria H. Borawska |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lactarius deliciosus DNA Replication DPPH Antineoplastic Agents Apoptosis RM1-950 Coprinus Mushroom 03 medical and health sciences chemistry.chemical_compound Astroglia 0302 clinical medicine Cell Line Tumor Humans MTT assay Viability assay Cantharellus Cell Proliferation Cancer Pharmacology biology Traditional medicine Chemistry Brain Neoplasms Basidiomycota Lycoperdon perlatum fungi General Medicine Cell Cycle Checkpoints Glioma biology.organism_classification Matrix Metalloproteinases Oxidative Stress 030104 developmental biology 030220 oncology & carcinogenesis Coprinus comatus Therapeutics. Pharmacology Agaricales Glioblastoma |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 133, Iss, Pp 111090-(2021) |
| ISSN: | 0753-3322 |
| Popis: | Cancer incidence rates are on the increase worldwide. The most common brain cancer in adults is glioblastoma. Currently available treatment modalities are limited and natural products such as mushrooms could enhance them. Apart from nutritional value, mushrooms are an excellent source of bioactive compounds and therefore could be used to treat various disorders. The aim of the study was to assess the anti-glioma potential of selected mushrooms on U87MG, LN-18 glioblastoma and SVGp12 normal human astroglial cell lines. The materials were Cantharellus cibarius, Coprinus comatus, Lycoperdon perlatum and Lactarius delicious. Aqueous, 70 % ethanol or 95 % ethanol extracts from mushrooms were used for analysis including assessment of antioxidant activity by DPPH assay, cell viability by MTT assay, DNA biosynthesis by thymidine incorporation assay, activity of metalloproteinase by gelatin zymography and cell cycle assay by flow cytometry. Mushroom extracts influenced the viability and DNA biosynthesis of cancer cells. Activity of ethanol mushroom extracts was stronger than that of aqueous extracts. Anti-glioma mechanism consisted in inhibition of cancer cell proliferation and induction of apoptosis associated with arrest of cells in subG1 or G2/M phase of cell cycle, and inhibition of metalloproteinases activity. Among investigated mushrooms, L. deliciosus and C. comatus showed the greatest anti-glioma potential. |
| Databáze: | OpenAIRE |
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