Tracking of Stem Cells from Human Exfoliated Deciduous Teeth Labeled with Molday ION Rhodamine-B during Periodontal Bone Regeneration in Rats

Autor: Nan Zhang, Li Xu, Hao Song, Chunqing Bu, Jie Kang, Chuanchen Zhang, Xiaofei Yang, Fabin Han
Rok vydání: 2021
Předmět:
Zdroj: International journal of stem cells.
ISSN: 2005-3606
Popis: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects.We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 μg Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging (MRI) was used to track SHED survival and migrationSHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked
Databáze: OpenAIRE