A member of the short-chain dehydrogenase/reductase (SDR) superfamily is a target of the ecdysone response in honey bee (Apis mellifera) caste development
| Autor: | Christine Hepperle, Karina R. Guidugli, Klaus Hartfelder |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
animal structures [SDV.BID]Life Sciences [q-bio]/Biodiversity Biology Reductase 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Polyphenism Complementary DNA Botany Gene expression Gene 030304 developmental biology [SDV.EE]Life Sciences [q-bio]/Ecology environment 0303 health sciences Differential display Ecdysteroid ecdysteroid fungi alcohol dehydrogenase differential display PCR caste polyphenism [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology 010602 entomology chemistry Biochemistry Insect Science [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies Apis mellifera Ecdysone |
| Zdroj: | Apidologie Apidologie, Springer Verlag, 2004, 35 (1), pp.37-47. ⟨10.1051/apido:2003068⟩ |
| ISSN: | 0044-8435 1297-9678 |
| DOI: | 10.1051/apido:2003068⟩ |
| Popis: | Many aspects in caste polyphenism result from hormonally controlled differential gene expression. A DDRT-PCR screen for ecdysteroid-regulated genes in ovaries revealed a set of ESTs coding for metabolic enzymes. For a cDNA encoding a short-chain dehydrogenase/reductase (SDR) we obtained the complete coding sequence (246 amino acids), revealing the protein motifs typical of insect SDRs. Its initially high expression in early fifth-instar larvae vanished in prepupae. Expression levels in worker larvae were higher than in queen larvae, suggesting negative regulation by the caste-specific ecdysteroid titer. This finding was confirmed by in vitro exposure of competent worker ovaries to makisterone A. In contrast to whole body RNA extracts, two SDR transcripts were detected in the ovaries. Both had their expression downregulated by makisterone A. Hormonal regulation and tissue-specific expression pattern makes this SDR an interesting enzyme for comparative molecular studies on social insect caste polyphenisms. Apis mellifera / differential display PCR / ecdysteroid / alcohol dehydrogenase / caste polyphenism |
| Databáze: | OpenAIRE |
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