| Autor: |
Liang, Guixian, Zhou, Chunyu, Jiang, Xiangxiang, Zhang, Yifan, Huang, Baofeng, Gao, Suwei, Kang, Zhixin, Ma, Dongyuan, Wang, Fengchao, Gottgens, Berthold, Wang, Hongmei, Han, Jing-Dong J, Liu, Feng |
| Přispěvatelé: |
Gottgens, Berthold [0000-0001-6302-5705], Apollo - University of Cambridge Repository |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.17863/cam.71399 |
| Popis: |
Macrophages play pivotal roles in immunity, hematopoiesis, and tissue homeostasis. In mammals, macrophages have been shown to originate from yolk-sac-derived erythro-myeloid progenitors and aorta-gonad-mesonephros (AGM)-derived hematopoietic stem cells. However, whether macrophages can arise from other embryonic sites remains unclear. Here, using single-cell RNA sequencing, we profile the transcriptional landscape of mouse fetal placental hematopoiesis. We uncover and experimentally validate that a CD44+ subpopulation of placental endothelial cells (ECs) exhibits hemogenic potential. Importantly, lineage tracing using the newly generated Hoxa13 reporter line shows that Hoxa13-labeled ECs can produce placental macrophages, named Hofbauer cell (HBC)-like cells. Furthermore, we identify two subtypes of HBC-like cells, and cell-cell interaction analysis identifies their potential roles in angiogenesis and antigen presentation, separately. Our study provides a comprehensive understanding of placental hematopoiesis and highlights the placenta as a source of macrophages, which has important implications for both basic and translational research. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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