| Autor: |
Ganesh-Kumar, Selvaraj, Hanqing, Wang, Yu, Zhang, Zhe, Tian, Wenbo, Chai, Huijie, Lu |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Science of The Total Environment. 821:153537 |
| ISSN: |
0048-9697 |
| Popis: |
The emergence of antibiotic resistance in retort to environmental pollutants during wastewater treatment still remains elusive. Here, we first to investigate the emergence of antibiotic resistance in an environmental non-pathogenic bacterium, Pseudoxanthomonas mexicana isolated from a lab-scale bioreactor treating wastewater containing streptomycin. The molecular mechanism of antibiotic resistance development was evaluated in its genomic, transcriptional, and proteomic levels. The streptomycin resistant (SR) strain showed strong resistance to streptomycin (MIC600 μg/mL) as well to sulfamethoxazole, ampicillin, and kanamycin (≥250 μg/mL). A 13.4 kb class-1-integron array consisting of a new arrangement of gene cassette (IS6100-sul1-aadA2-catB3-aacA1-2-aadB-int1-IS256-int) linked with Tn5393c transposon was identified in the SR strain, which has only been reported in clinical pathogens so far. iTRAQ-LC-MS/MS proteomics revealed 22 up-regulated proteins in the SR strain growing under 100 mg L |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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