TGF-β1 Promotes Lymphangiogenesis during Peritoneal Fibrosis
| Autor: | Yoshifumi Takei, Masashi Mizuno, Ryohei Hattori, Enyu Imai, Yasuhiko Ito, Fumiko Nagura, Shoichi Maruyama, Hiroshi Kinashi, Seiichi Matsuo, Tomohiro Mizuno, Takeshi Terabayashi, Yukihiro Noda, Yoshihisa Matsukawa, Ryosuke Nishio, Yasuhiro Suzuki, Hayato Nishimura |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Vascular Endothelial Growth Factor C Peritonitis Cell Line Rats Sprague-Dawley Transforming Growth Factor beta1 Peritoneum Downregulation and upregulation Fibrosis medicine Animals Humans Lymphangiogenesis Peritoneal Fibrosis Aged Lymphatic Vessels Aged 80 and over Membrane Glycoproteins business.industry Chlorhexidine General Medicine Middle Aged medicine.disease Rats Up-Regulation Basic Research Hyaluronan Receptors medicine.anatomical_structure Lymphatic system Vascular endothelial growth factor C Nephrology Immunology Cancer research Female business Peritoneal Dialysis |
| Zdroj: | Journal of the American Society of Nephrology. 24:1627-1642 |
| ISSN: | 1046-6673 |
| DOI: | 10.1681/asn.2012030226 |
| Popis: | Peritoneal fibrosis (PF) causes ultrafiltration failure (UFF) and is a complicating factor in long-term peritoneal dialysis. Lymphatic reabsorption also may contribute to UFF, but little is known about lymphangiogenesis in patients with UFF and peritonitis. We studied the role of the lymphangiogenesis mediator vascular endothelial growth factor-C (VEGF-C) in human dialysate effluents, peritoneal tissues, and peritoneal mesothelial cells (HPMCs). Dialysate VEGF-C concentration correlated positively with the dialysate-to-plasma ratio of creatinine (D/P Cr) and the dialysate TGF-β1 concentration. Peritoneal tissue from patients with UFF expressed higher levels of VEGF-C, lymphatic endothelial hyaluronan receptor-1 (LYVE-1), and podoplanin mRNA and contained more lymphatic vessels than tissue from patients without UFF. Furthermore, mesothelial cell and macrophage expression of VEGF-C increased in the peritoneal membranes of patients with UFF and peritonitis. In cultured mesothelial cells, TGF-β1 upregulated the expression of VEGF-C mRNA and protein, and this upregulation was suppressed by a TGF-β type I receptor (TGFβR-I) inhibitor. TGF-β1–induced upregulation of VEGF-C mRNA expression in cultured HPMCs correlated with the D/P Cr of the patient from whom the HPMCs were derived (P |
| Databáze: | OpenAIRE |
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