| Autor: |
Shen, Yu, Zheng, Bin, Sun, Hao, Wu, Songrui, Fan, Jiyuan, Ding, Jianzu, Gao, Meng, Kong, Qingming, Lou, Di, Ding, Haojie, Zhuo, Xunhui, Lu, Shaohong |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.23529043 |
| Popis: |
Additional file 1: Table S1. The primers used in this study. Table S2. This table shows the oocysts in the feces of cats from 1 to 10 days after being infected with RHΔompdcΔuprt strain. Figure S1. The diagram of knocking out. (a) Schematic illustration of knocking out uprt by homologous gene replacement in RHΔku80Δhxgprt strain. (b) Schematic showing deletion of hxgprt gene by insertion of a uprt5’utr-3’utr into hxgprt gene. (c) Diagram illustrating the deletion of ompdc in RHΔuprtΔhxgprt to make the double mutant RHΔuprtΔompdc::HXGPRT by CRISPR/Cas9-mediated homologous gene replacement. Figure S2. Intracellular proliferation of OMPDC-UPRT deletion mutant under the fluorescence microscope. (a) 24 hours of intracellular proliferation. (b) 48 hours of intracellular proliferation. Figure S3. Invasion and attachment assay for RHΔku80 and RHΔompdcΔuprt strains. (a) The invasion and attachment of T. gondii RHΔku80 and RHΔompdcΔuprt were evaluated by indirect immunofluorescence assay. (b) The analysis of the invasion and attachment. Figure S4. The levels of cytokines in the serum of mice injected with RHΔompdcΔuprt. The levels of IFN-γ (a) and IL-12 (b) were measured by flow cytometer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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