Quantitative comparative immunohistology
| Autor: | Kurt Lange, Gerhard Treser, Martin Semar |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Biopsy
Clinical Biochemistry Analytical chemistry Fluorescent Antibody Technique law.invention Glomerulonephritis law Fluorometer Microtome Methods Animals Humans Sodium Hydroxide Fluorometry Chromatography Chemistry Immune Sera Biochemistry (medical) Phosphate buffered saline Fluorescence Rats Standard curve Tissue sections Quantitative assay Immunoglobulin G Rabbits Protein Binding |
| Zdroj: | Clinical chemistry. 15(6) |
| ISSN: | 0009-9147 |
| Popis: | A quantitative assay for the determination of fluorescence of tissue sections stained with fluorescein-labeled antibodies using a fluorometer is described. Sequential microtome sections were stained with labeled antiserums, washed repeatedly with buffered saline, and digested with 20% (w/v) NaOH at 60° for 12 hr. The fluorescence of the resultant solution is then read in a fluorometer. There is similarity between sequential sections of the same biopsy specimen. Specific blocking procedures reduce the readings of the fluorescence markedly. By comparison with values obtained simultaneously from standard curves of the labeled antiserums used, the amount of bound antibody or specific proteins can be determined quantitatively. |
| Databáze: | OpenAIRE |
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