Self-differentiation of human fetal lung organ culture: the role of prostaglandins PGE2 and PGF2 alpha
| Autor: | Anne Hallas, Rodney W. Kelly, Merete Giles, Robert Hume, David Cossar, Margaret Gourlay, Jeanne E. Bell |
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| Rok vydání: | 1991 |
| Předmět: |
Necrosis
Cell Survival Cellular differentiation Indomethacin Alpha (ethology) Prostaglandin Endogeny Biology Organ culture Dinoprost Dinoprostone Epithelium Andrology Tissue culture chemistry.chemical_compound Fetus Organ Culture Techniques medicine Humans Lung Dose-Response Relationship Drug Cell Differentiation Epithelial Cells Cell Biology chemistry Immunology lipids (amino acids peptides and proteins) medicine.symptom |
| Zdroj: | Experimental cell research. 194(1) |
| ISSN: | 0014-4827 |
| Popis: | Addition of PGE2, but not PGF2 alpha, to fetal lung organ cultures accelerates the process of self-differentiation with increased dilatation of terminal airsacs and differentiation of the epithelial lining. Indomethacin reduces the endogenous production by organ cultures of PGE2, PGF2 alpha, 13,14-dihydro-15-keto-PGE2, and 13,14-dihydro-15-keto-PGF2 alpha and retards the process of self-differentiation. Prolonged exposure of cultures to indomethacin results in cell necrosis. Indomethacin inhibition of self-differentiation can be reversed and accelerated by the addition of PGE2. Addition of PGF2 alpha in the presence of indomethacin prevents indomethacin-associated cell necrosis but does not accelerate dilatation or differentiation beyond that of cultures in sera-free media without additions. We propose that the endogenous production of PGE2 is a key process in the mechanism of self-differentiation of human fetal lung in organ culture. |
| Databáze: | OpenAIRE |
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