Effect of CFIm68 knockdown on RNA polymerase II transcription
Autor: | Tellier, M, Hardy, J, Norbury, C, Murphy, S |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
mRNA Cleavage and Polyadenylation Factors
0303 health sciences Transcription Genetic lcsh:R lcsh:Medicine General Medicine Data Note General Biochemistry Genetics and Molecular Biology 03 medical and health sciences HEK293 Cells 0302 clinical medicine lcsh:Biology (General) Gene Knockdown Techniques Pol II pausing Humans RNA polymerase II CFIm68 lcsh:Science (General) Transcription Cleavage and polyadenylation factors lcsh:QH301-705.5 030217 neurology & neurosurgery 030304 developmental biology lcsh:Q1-390 |
Zdroj: | BMC Research Notes, Vol 12, Iss 1, Pp 1-4 (2019) BMC Research Notes |
ISSN: | 1756-0500 |
DOI: | 10.1186/s13104-019-4582-8 |
Popis: | Objectives Transcription of eukaryotic protein-coding genes by RNA polymerase II (pol II) is highly regulated at initiation, elongation and termination. Transcription is also coordinated with co-transcriptional processing of the emerging pre-mRNA by capping, splicing, and cleavage and polyadenylation. Polyadenylation (poly(A)) site recognition, which defines the end of the mRNA, relies on the cleavage and polyadenylation (CPA) complex. It was previously observed that knocking-down proteins of the CPA complex affects not only recognition of the poly(A) site but also results in increased pausing of pol II at the beginning of genes. This finding suggests that the CPA complex plays a role in regulating pol II turnover after transcription initiation. Data description To explore this possibility, we knocked-down a subunit of the cleavage factor I (CFIm), CFIm68, which is part of the CPA complex and involved in alternative polyadenylation, and performed pol II ChIP-seq in absence or presence of a transcription elongation inhibitor. In addition, we performed pol II ChIP-qPCR on a subset of protein coding genes after knocking down CFIm68. |
Databáze: | OpenAIRE |
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