Fructose and stress induce opposite effects on lipid metabolism in the visceral adipose tissue of adult female rats through glucocorticoid action

Autor: Jelena Nestorov, Gordana Matić, Ivana Elaković, Sanja Kovačević
Rok vydání: 2016
Předmět:
0301 basic medicine
medicine.medical_specialty
Medicine (miscellaneous)
Adipose tissue
030209 endocrinology & metabolism
Fructose
Intra-Abdominal Fat
Biology
03 medical and health sciences
chemistry.chemical_compound
Receptors
Glucocorticoid
0302 clinical medicine
Glucocorticoid receptor
Stress
Physiological
Internal medicine
11-beta-Hydroxysteroid Dehydrogenase Type 1
medicine
Animals
Lipolysis
Chronic stress
Obesity
Rats
Wistar
Glucocorticoids
Nutrition and Dietetics
Lipid metabolism
Sterol Esterase
Lipid Metabolism
Diet
Rats
PPAR gamma
Disease Models
Animal
Lipoprotein Lipase
030104 developmental biology
Endocrinology
Gene Expression Regulation
chemistry
Lipogenesis
Female
Fatty Acid Synthases
Corticosterone
Phosphoenolpyruvate Carboxykinase (ATP)
Glucocorticoid
Acetyl-CoA Carboxylase
medicine.drug
Zdroj: European Journal of Nutrition. 56:2115-2128
ISSN: 1436-6215
1436-6207
DOI: 10.1007/s00394-016-1251-8
Popis: Daily exposure to stress and excessive fructose intake coincides with the growing rate of obesity and related disorders, to which women are more prone than men. Glucocorticoids, the main regulators of energy balance and response to stress, have been associated with the development of metabolic disturbances. The aim of the present study was to examine the effects of fructose overconsumption and/or chronic stress on glucocorticoid signalization and lipid metabolism in female rat adipose tissue. We examined the effects of fructose-enriched diet and chronic unpredictable stress, separately and in combination, on glucocorticoid signaling in terms of 11β-hydroxysteroid dehydrogenase 1 (HSD1)-catalyzed corticosterone regeneration, glucocorticoid receptor (GR) intracellular distribution, hormone binding and transcriptional regulation of genes involved in lipolysis (hormone-sensitive lipase) and lipogenesis (lipoprotein lipase, acetyl-CoA carboxylase, fatty acid synthase and phosphoenolpyruvate carboxykinase) in the visceral adipose tissue (VAT) of adult female rats. Additionally, the nuclear level of the peroxisomal proliferator-activated receptor γ (PPARγ) was analyzed. The combination of stress and fructose-enriched diet led to an elevation in HSD1 expression and intracellular corticosterone concentration, whereas GR nuclear accumulation was enhanced after separate treatments. Furthermore, fructose was shown to induce the expression of all examined lipogenic genes and nuclear accumulation of PPARγ, thereby stimulating adipogenesis, while stress upregulated HSL, reducing the adipose tissue mass regardless of fructose consumption. Prolonged overconsumption of fructose and chronic exposure to stress promote opposite effects on lipid metabolism in the VAT of adult female rats and suggest that these effects could be mediated by glucocorticoids.
Databáze: OpenAIRE
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