Maternal Larp6 controls oocyte development, chorion formation and elevation
| Autor: | Roland A. Fleck, Andrew H Hibbert, Katherine Exelby, Hoi Ting A Hau, Jessica Nevarez-Mejia, Natalie J Wood, Tapan G. Pipalia, Maria Dermit, Faraz K. Mardakheh, Victoria C Williams-Ward, Simon M. Hughes, Oluwaseun Ogundele, Clinton A. L. Monfries, M. Alejandra Carbajal, Maria R. Conte |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Oocyte Mutant Autoantigens Larp6 Oogenesis 0302 clinical medicine Maternal effect Cell Movement Lectins Zona pellucida Zebrafish Gene Editing 0303 health sciences Genome Homozygote Gene Expression Regulation Developmental Embryo Chorion Cell biology Phenotype medicine.anatomical_structure Ribonucleoproteins 030220 oncology & carcinogenesis embryonic structures Female Collagen Research Article endocrine system Heterozygote Genotype Knockout Biology 03 medical and health sciences Ciliogenesis medicine Animals Molecular Biology Gene Zona Pellucida Cell Proliferation 030304 developmental biology Mass spectrometry Gene Expression Profiling Egg Proteins Wild type Oocyte activation Mutation Oocytes Developmental Biology |
| Zdroj: | Development (Cambridge, England) |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.187385 |
| Popis: | La-related protein 6 (Larp6) is a conserved RNA-binding protein found across eukaryotes that has been suggested to regulate collagen biogenesis, muscle development, ciliogenesis, and various aspects of cell proliferation and migration. Zebrafish have two Larp6 family genes: larp6a and larp6b. Viable and fertile single and double homozygous larp6a and larp6b zygotic mutants revealed no defects in muscle structure, and were indistinguishable from heterozygous or wild-type siblings. However, larp6a mutant females produced eggs with chorions that failed to elevate fully and were fragile. Eggs from larp6b single mutant females showed minor chorion defects, but chorions from eggs laid by larp6a;larp6b double mutant females were more defective than those from larp6a single mutants. Electron microscopy revealed defective chorionogenesis during oocyte development. Despite this, maternal zygotic single and double mutants were viable and fertile. Mass spectrometry analysis provided a description of chorion protein composition and revealed significant reductions in a subset of zona pellucida and lectin-type proteins between wild-type and mutant chorions that paralleled the severity of the phenotype. We conclude that Larp6 proteins are required for normal oocyte development, chorion formation and egg activation. Summary: Genome-edited larp6a and larp6b double-knockout zebrafish appear morphologically and behaviourally wild type and are fertile. Larp6 proteins are, however, required maternally for normal oocyte development and chorion formation and elevation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|