Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays
| Autor: | Lekh Raj Juneja, Young Il Kim, Hiroko Nakayama, Djong-Chi Chu, Yasuhito Shimada, Izumi Matsuoka, Liqing Zang, Norihiro Nishimura, Kanae Hata |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
030309 nutrition & dietetics
Drug Evaluation Preclinical Pharmaceutical Science Green tea extract Biology Pharmacology Article Nobiletin metabolic syndrome Analytical Chemistry drug discovery lcsh:QD241-441 Mice 03 medical and health sciences chemistry.chemical_compound lcsh:Organic chemistry In vivo 3T3-L1 Cells Adipocytes medicine Animals Physical and Theoretical Chemistry Zebrafish Cells Cultured 030304 developmental biology Biological Products 0303 health sciences Adipogenesis diabetes Drug discovery screening Organic Chemistry fungi 3T3-L1 biology.organism_classification medicine.disease chemistry Chemistry (miscellaneous) Molecular Medicine Anti-Obesity Agents Metabolic syndrome |
| Zdroj: | Molecules Volume 25 Issue 24 Molecules, Vol 25, Iss 5840, p 5840 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25245840 |
| Popis: | (1) Background: The obesity epidemic has been drastically progressing in both children and adults worldwide. Pharmacotherapy is considered necessary for its treatment. However, many anti-obesity drugs have been withdrawn from the market due to their adverse effects. Instead, natural products (NPs) have been studied as a source for drug discovery for obesity, with the goal of limiting the adverse effects. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. However, the evidence for zebrafish as an anti-obesity drug screening model are still limited. (2) Methods: We performed anti-adipogenic testing using the juvenile zebrafish obesogenic test (ZOT) and mouse 3T3-L1 preadipocytes using the focused NP library containing 38 NPs and compared their results. (3) Results: Seven and eleven NPs reduced lipid accumulation in zebrafish visceral fat tissues and mouse adipocytes, respectively. Of these, five NPs suppressed lipid accumulation in both zebrafish and 3T3-L1 adipocytes. We confirmed that these five NPs (globin-digested peptides, green tea extract, red pepper extract, nobiletin, and Moringa leaf powder) exerted anti-obesity effects in diet-induced obese adult zebrafish. (4) Conclusions: ZOT using juvenile fish can be a high-throughput alternative to ZOT using adult zebrafish and can be applied for in vivo screening to discover novel therapeutics for visceral obesity and potentially also other disorders. |
| Databáze: | OpenAIRE |
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