Taxonomic and Functional Diversity of a Quercus pyrenaica Willd. Rhizospheric Microbiome in the Mediterranean Mountains
| Autor: | Nicolas Toro, Pablo José Villadas Latorre, José Francisco Cobo Díaz, Susannah Tringe, Antonio José Fernández-González, Manuel Fernandez-Lopez, Antonio J Fernández-González |
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| Přispěvatelé: | Junta de Andalucía, Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España), Organismo Autónomo Parques Nacionales (España), European Commission |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Life on Land Plant Biology rhizosphere metabolism Quercus pyrenaica 03 medical and health sciences Mediterranean forests Altitude melojo-oak Soil pH Botany Tree canopy Rhizosphere metagenomics biology Ecology Forestry Sciences Forestry Vegetation biogeochemical cycles lcsh:QK900-989 microbial functional diversity biology.organism_classification 030104 developmental biology Microbial population biology Metagenomics lcsh:Plant ecology |
| Zdroj: | Forests, Vol 8, Iss 10, p 390 (2017) Forests, vol 8, iss 10 Forests; Volume 8; Issue 10; Pages: 390 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1999-4907 |
| Popis: | Altitude significantly affects vegetation growth and distribution, including the developmental stages of a forest. We used shotgun Illumina sequencing to analyze microbial community composition and functional potential in melojo-oak (Quercus pyrenaica Willd.) rhizospheric soil for three different development stages along an altitudinal gradient: (a) a low altitude, non-optimal site for forest maintenance; (b) an intermediate altitude, optimal site for a forest; and (c) a high altitude, expansion site with isolated trees but without a real forest canopy. We observed that, at each altitude, the same microbial taxa appear both in the taxonomic analysis of the whole metagenome and in the functional analysis of the methane, sulfur and nitrogen metabolisms. Although there were no major differences at the functional level, there were significant differences in the abundance of each taxon at the phylogenetic level between the rhizospheres of the forest (low and intermediate altitudes) and the expansion site. Proteobacteria and Actinobacteria were the most differentially abundant phyla in forest soils compared to the expansion site rhizosphere. Moreover, Verrucomicrobia, Bacteroidetes and Nitrospirae phyla were more highly represented in the non-forest rhizosphere. Our study suggests that rhizospheric microbial communities of the same tree species may be affected by development stage and forest canopy cover via changes in soil pH and the C/N ratio. This work was supported by research grants including ERDF (European Regional Development Funds): P08-CVI-03549 from Consejería de Innovación, Ciencia y Empresa from Junta de Andalucía, OAPN 021/2007 from Organismo Autónomo Parques Nacionales (Spanish Ministry of Environment) and 20134R069-RECUPERA 2020 from the Spanish Ministerio de Economía y Competitividad and CSIC. We would like to thank the authorities of the Sierra Nevada National Park for the access, facilities and soil sampling, and also Edward Kirton (JGI, California) for technical assistance with bioinformatics. JFCD was awarded a postdoctoral contract from Junta de Andalucía, and AJFG was awarded a postdoctoral contract from RECUPERA 2020. The work of the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. |
| Databáze: | OpenAIRE |
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