Retrograde axonal transport of neurotensin in the dopaminergic nigrostriatal pathway in the rat
| Autor: | C. Malgouris, P.M. Laduron, M. N. Castel, Jean-Charles Blanchard |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Dopamine Neuropeptide Nigrostriatal pathway Substantia nigra Biology Axonal Transport complex mixtures chemistry.chemical_compound Neural Pathways medicine Animals Receptor Neurotensin Brain Chemistry musculoskeletal neural and ocular physiology General Neuroscience digestive oral and skin physiology Dopaminergic Rats Inbred Strains Axons Corpus Striatum Rats Substantia Nigra Kinetics medicine.anatomical_structure nervous system chemistry Axoplasmic transport Autoradiography Kelatorphan Neuroscience hormones hormone substitutes and hormone antagonists |
| Zdroj: | Neuroscience. 36:425-430 |
| ISSN: | 0306-4522 |
| Popis: | Although the existence of receptor transport has been clearly demonstrated in peripheral nerves, there is no clear cut evidence in the brain of such a process for neuropeptide receptors. Because of the localization of neurotensin receptors on dopaminergic terminals, the dopaminergic nigrostriatal pathway appears to be the system of choice for studying the axonal transport of neuropeptide receptors in the brain. When labelled neurotensin was injected into the rat striatum, a delayed accumulation of radioactivity in the ipsilateral substantia nigra was observed about 2 h after injection. An essential requirement to clearly observe this phenomenon was the pretreatment of animals with kelatorphan in order to prevent the labelled neurotensin degradation. The appearance of this labelling was prevented by injection of an excess of unlabelled neurotensin or of neurotensin 8-13, an active neurotensin fragment, but not by neurotensin 1-8, which had no affinity for neurotensin receptors. This process was saturable, microtubule-dependent and occurred only in mesostriatal and nigrostriatal dopaminergic neurons as identified after 6-hydroxydopamine lesion and by autoradiography. These results demonstrate that neurotensin was retrogradely transported by a process involving neurotensin receptors. The retrograde transport of receptor-bound neuropeptide may represent an important dynamic process which conveys information molecules from the synapse towards the cell body. |
| Databáze: | OpenAIRE |
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