A novel extracellular low-temperature active phytase from Bacillus aryabhattai RS1 with potential application in plant growth
| Autor: | Moushree Pal Roy, Shilpi Ghosh, Subhabrata Datta |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bacillus Germination 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Extracellular Peptide sequence chemistry.chemical_classification Rhizosphere 6-Phytase biology Temperature Hydrogen-Ion Concentration biology.organism_classification Phosphate Cicer Cold Temperature 030104 developmental biology Enzyme chemistry Biochemistry Phytase Specific activity Biotechnology |
| Zdroj: | Biotechnology progress. 33(3) |
| ISSN: | 1520-6033 |
| Popis: | Bacillus aryabhattai RS1 isolated from rhizosphere produced an extracellular, low temperature active phytase. The cultural conditions for enzyme production were optimized to obtain 35 U mL-1 of activity. Purified phytase had specific activity and molecular weight of 72.97 U mg-1 and ∼40 kDa, respectively. The enzyme was optimally active at pH 6.5 and 40°C and was highly specific to phytate. It exhibited higher catalytic activity at low temperature, retaining over 40% activity at 10°C. Phytase was more thermostable in presence of Ca2+ ion and retained 100% residual activity on preincubation at 20-50°C for 30 min. Partial phytase encoding gene, phyB (816 bp) was cloned and sequenced. The encoded amino acid sequence (272 aa) contained two conserved motifs, DA[A/T/E]DDPA[I/L/V]W and NN[V/I]D[I/L/V]R[Y/D/Q] of β-propellar phytase and had lower sequence homology with other Bacillus phytases, indicating its novelty. Phytase and the bacterial inoculum were effective in improving germination and growth of chickpea seedlings under phosphate limiting condition. Moreover, the potential applications of the enzyme with relatively high activity at lower temperatures (20-30°C) could also be extended to aquaculture and food processing. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:633-641, 2017. |
| Databáze: | OpenAIRE |
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