Antioxidant properties and inhibitory effects of Satureja khozestanica essential oil on LDL oxidation induced–CuSO4 in vitro
| Autor: | Habibolah Nazem, Ali Khosrowbeygi, farshid Ghazanfari, Hassan Ahmadvand, Shahrokh Bagheri, Narges Jafari, Reza Haji Hosseini |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Copper Sulfate Antioxidant food.ingredient medicine.medical_treatment Satureja Biochemistry Genetics and Molecular Biology (miscellaneous) Antioxidants law.invention chemistry.chemical_compound food law Malondialdehyde Oils Volatile medicine Humans Food science IC50 Essential oil Ascorbic acid In vitro Lipoproteins LDL chemistry Biochemistry Low-density lipoprotein Original Article Oxidation-Reduction |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine. 3:22-27 |
| ISSN: | 2221-1691 |
| DOI: | 10.1016/s2221-1691(13)60018-0 |
| Popis: | Objective: To assess various antioxidative activities of Satureja khozestanica essential oil (SKE) and its effect on oxidation of low density lipoprotein (LDL) induced by CuSO4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde (MDA). Methods: The formation of conjugated dienes, lag time and MDA were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE. Also total antioxidant activity and free radical scavenging of SKE were determinated. Results: It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation. The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200 μg/mL. Total antioxidant capacity of SKE was (3.20依0.40) nmol of ascorbic acid equivalents/g SKE. The SKE showed remarkable scavenging activity on 2, 2-diphenyl- picrylhydrazyl, IC50 (5.30依0.11) ng/mL. Conclusions: This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications. |
| Databáze: | OpenAIRE |
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