Changes in Serum Iron and Leukocyte mRNA Levels of Genes Involved in Iron Metabolism in Amateur Marathon Runners-Effect of the Running Pace
| Autor: | Jedrzej Antosiewicz, Katarzyna Anczykowska, Małgorzata Żychowska, Wojciech Ratkowski, Agata Grzybkowska, Piotr Aschenbrenner, Iwona Bonisławska |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Gene Expression Running chemistry.chemical_compound Hemoglobins 0302 clinical medicine TFRC Gene expression Leukocytes iron metabolism Genetics (clinical) medicine.diagnostic_test Myoglobin RNA-Binding Proteins Middle Aged DNA-Binding Proteins C-Reactive Protein Serum iron marathon runners Oxidoreductases Adult medicine.medical_specialty lcsh:QH426-470 Iron Physical exercise Biology Article 03 medical and health sciences PCBP1 Internal medicine Genetics medicine Humans RNA Messenger PCBP2 Messenger RNA ferritin 030229 sport sciences Metabolism Ferritin lcsh:Genetics 030104 developmental biology Endocrinology chemistry Athletes Apoferritins Ferritins biology.protein Hemoglobin |
| Zdroj: | Genes Volume 10 Issue 6 Genes, Vol 10, Iss 6, p 460 (2019) |
| ISSN: | 2073-4425 |
| Popis: | Iron is essential for physical activity due to its role in energy production pathways and oxygen transportation via hemoglobin and myoglobin. Changes in iron-related biochemical parameters after physical exercise in athletes are of substantial research interest, but molecular mechanisms such as gene expression are still rarely tested in sports. In this paper, we evaluated the mRNA levels of genes related to iron metabolism (PCBP1, PCBP2, FTL, FTH, and TFRC) in leukocytes of 24 amateur runners at four time points: before, immediately after, 3 h after, and 24 h after a marathon. We measured blood morphology as well as serum concentrations of iron, ferritin, and C-reactive protein (CRP). Our results showed significant changes in gene expression (except for TFRC), serum iron, CRP, and morphology after the marathon. However, the alterations in mRNA and protein levels occurred at different time points (immediately and 3 h post-run, respectively). The levels of circulating ferritin remained stable, whereas the number of transcripts in leukocytes differed significantly. We also showed that running pace might influence mRNA expression. Our results indicated that changes in the mRNA of genes involved in iron metabolism occurred independently of serum iron and ferritin concentrations. |
| Databáze: | OpenAIRE |
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