| Autor: |
Xuan Li, Jingjun Lyu, Ran Li, Vaibhav Jain, Yuntian Shen, Ángela del Águila, Ulrike Hoffmann, Huaxin Sheng, Wei Yang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of neuroinflammation. 19(1) |
| ISSN: |
1742-2094 |
| Popis: |
Background Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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