| Autor: |
Emmanuel Camors, Patricia A. Powers, Héctor H. Valdivia, Yan-Ting Zhao, Randall Loaiza, Francisco J. Alvarado |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Biophysical Journal. 106(2) |
| ISSN: |
0006-3495 |
| DOI: |
10.1016/j.bpj.2013.11.665 |
| Popis: |
We previously showed that ablation of the cardiac Ca2+ release channel/ryanodine receptor (RyR2) phosphorylation at Ser2808 (RyR2-S2808A) has no functional impact on cardiac performance and/or heart failure progression. In this study, we investigated whether this lack of effect may be due to compensatory mechanisms involving altered phosphorylation of the nearby Ser2814 CaMKII phospho-site. We generated a new mouse model where Ser2808 and Ser2814 were genetically replaced by Alanine (RyR2-S2808A/S2814A). Anesthetized RyR2-S2808A/S2814A mice have normal ejection fraction at baseline compared to wild type (WT) mice (55.1±2.6% vs. 55.9±2.4%, respectively, n=7/group) but exhibit ∼20% increase in heart rate (553±33 vs. 460±28 bpm, p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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