Stability indicating methods for the determination of diloxanide furoate
| Autor: | Nagiba Y Hasan, Nour E Wagieh, Mohamed A Elkawy, Badr E Elzeany |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Clinical Biochemistry
Analytical chemistry Pharmaceutical Science High-performance liquid chromatography Dosage form Analytical Chemistry chemistry.chemical_compound Drug Stability Spectrophotometry Drug Discovery medicine Amebicides Furans Chromatography High Pressure Liquid Spectroscopy Second derivative Chloroform Chromatography medicine.diagnostic_test Silica gel Reproducibility of Results Reversed-phase chromatography Reference Standards chemistry Indicators and Reagents Spectrophotometry Ultraviolet Chromatography Thin Layer Methanol Densitometry Tablets |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 28:187-197 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/s0731-7085(01)00583-0 |
| Popis: | Five new selective, precise and accurate methods are described for the determination of diloxanide furoate (DI) in presence of its degradation products. Method A utilizes the first and second derivative spectrophotometry at 270 and 280 nm, respectively. Method B is a RSD(1) spectrophotometric method based on the simultaneous use of the first derivative of ratio spectra and measurement at 270 nm. Method C is a pH-induced difference spectrophotometry using UV measurement at 295 nm. Method D is a densitometric one, after separation on silica gel plate using chloroform: methanol as mobile phase and the spots were scanned at 258 nm. Method E is reversed phase high performance liquid chromatography using methanol: water (80:20% v/v) as mobile phase at a flow rate of 1 ml/min and UV detection at 258 nm. Regression analysis showed good correlation in the concentration ranges 5-30, 5-25, 10-40 microg/ml, 100-500 ng/spot, 2-50 microg/ml with percentage recoveries of 99.92+/-0.56 and 99.79+/-0.47, 99.23+/-0.38, 99.96+/-0.06, 99.03+/-0.51, 98.81+/-0.68 for methods A, B, C, D and E, respectively. These methods are suitable as stability indicating methods for the determination of DI in presence of its degradation products either in bulk powder or in pharmaceutical formulations. |
| Databáze: | OpenAIRE |
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