The Rosmarinus Bioactive Compound Carnosic Acid Is a Novel PPAR Antagonist That Inhibits the Browning of White Adipocytes

Autor: N Gautier, Nathalie Rochet, Gérard Ailhaud, Ez-Zoubir Amri, Cécilia Colson, Pierre-Louis Batrow, Franck Peiretti
Přispěvatelé: Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-17-CE14-0015,HepAdialogue,Impact du métabolisme des acides gras des tissus adipeux et du foie sur l'insulinorésistance(2017), peiretti, franck, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Lipolysis
[SDV]Life Sciences [q-bio]
Adipocytes
White
Peroxisome Proliferator-Activated Receptors
Peroxisome proliferator-activated receptor
Adipose tissue
conversion. Conflict of interest statement
adipocyte
Article
Rosiglitazone
Mice
03 medical and health sciences
chemistry.chemical_compound
Transactivation
0302 clinical medicine
Adipocyte
Animals
Humans
Adipocytes
Beige
conversion
Receptor
lcsh:QH301-705.5
carnosic acid
030304 developmental biology
chemistry.chemical_classification
browning
0303 health sciences
Thermogenesis
Carnosic acid
antagonist
General Medicine
Peroxisome
Rosmarinus
Bioactive compound
3. Good health
Cell biology
[SDV] Life Sciences [q-bio]
Adipocytes
Brown
HEK293 Cells
Gene Expression Regulation
lcsh:Biology (General)
chemistry
030220 oncology & carcinogenesis
Abietanes
PPA
Biomarkers
Zdroj: Cells
Volume 9
Issue 11
Cells, Vol 9, Iss 2433, p 2433 (2020)
Cells, 2020, 9 (11), pp.2433. ⟨10.3390/cells9112433⟩
Cells, MDPI, 2020, 9 (11), pp.2433. ⟨10.3390/cells9112433⟩
ISSN: 2073-4409
DOI: 10.3390/cells9112433
Popis: International audience; Thermogenic brown and brite adipocytes convert chemical energy from nutrients into heat. Therapeutics that regulate brown adipocyte recruitment and activity represent interesting strategies to control fat mass such as in obesity or cachexia. The peroxisome proliferator-activated receptor (PPAR) family plays key roles in the maintenance of adipose tissue and in the regulation of thermogenic activity. Activation of these receptors induce browning of white adipocyte. The purpose of this work was to characterize the role of carnosic acid (CA), a compound used in traditional medicine, in the control of brown/brite adipocyte formation and function. We used human multipotent adipose-derived stem (hMADS) cells differentiated into white or brite adipocytes. The expression of key marker genes was determined using RT-qPCR and western blotting. We show here that CA inhibits the browning of white adipocytes and favors decreased gene expression of thermogenic markers. CA treatment does not affect β-adrenergic response. Importantly, the effects of CA are fully reversible. We used transactivation assays to show that CA has a PPARα/γ antagonistic action. Our data pinpoint CA as a drug able to control PPAR activity through an antagonistic effect. These observations shed some light on the development of natural PPAR antagonists and their potential effects on thermogenic response.
Databáze: OpenAIRE
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