Quantification of Calcyclin and Heat Shock Protein 90 in Sera from Women with and without Preeclampsia by Mass Spectrometry
| Autor: | Eric A.P. Steegers, Marcel van der Weiden, Christoph Stingl, Thierry P P van den Bosch, Theo M. Luider, Caroline B van den Berg, Coşkun Güzel, Johannes J. Duvekot, Régine P.M. Steegers-Theunissen |
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| Přispěvatelé: | Neurology, Obstetrics & Gynecology, Pathology |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Proteomics 0301 basic medicine placenta PRM Clinical Biochemistry FFPE Immunofluorescence Mass Spectrometry S100 Calcium Binding Protein A6 Preeclampsia preeclampsia Andrology Pathogenesis 03 medical and health sciences Pre-Eclampsia Pregnancy Heat shock protein Placenta medicine HSP90 Humans Clinical significance HSP90 Heat-Shock Proteins immunofluorescence Research Articles S100A6 030102 biochemistry & molecular biology biology medicine.diagnostic_test business.industry medicine.disease Hsp90 Trophoblasts 030104 developmental biology medicine.anatomical_structure Case-Control Studies biology.protein Female business serum Research Article |
| Zdroj: | Proteomics. Clinical Applications Proteomics Clinical Applications, 13(3):1800181. Wiley-VCH |
| ISSN: | 1862-8354 1862-8346 |
| DOI: | 10.1002/prca.201800181 |
| Popis: | Purpose: The objective of present study is to determine serum levels and placental distribution of two interacting proteins calcyclin and heat shock protein 90 in preeclampsia. Experimental design: Maternal serum levels of calcyclin and heat shock protein 90 are compared throughout pregnancy from the first trimester till term among women with preeclampsia (n = 43) and age-matched normotensive pregnant controls (n = 46). A serum-based 2D LC-MS assay using Parallel Reaction Monitoring is applied to quantify both calcyclin and heat shock protein 90. Results: Serum levels of calcyclin are significantly lower in patients with preeclampsia in the second trimester of pregnancy as compared to controls (p |
| Databáze: | OpenAIRE |
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