Autophagic and proteolytic processes in the Harderian gland are modulated during the estrous cycle
| Autor: | Marina García-Macia, Yaiza Potes, José Antonio Boga, David de Gonzalo-Calvo, Susana Rodríguez-González, Adrian Rubio-González, Ana Coto-Montes, B. de Luxán-Delgado |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Histology Porphyrins medicine.drug_class Proteolysis Hamster Estrous Cycle medicine.disease_cause Harderian gland Porphyrias Pregnancy Internal medicine medicine Autophagy Animals Humans Molecular Biology Estrous cycle medicine.diagnostic_test biology Mesocricetus Harderian Gland Estrogens Cell Biology biology.organism_classification Pregnancy Complications Medical Laboratory Technology Endocrinology Estrogen Female Oxidative stress |
| Zdroj: | Histochemistry and cell biology. 141(5) |
| ISSN: | 1432-119X |
| Popis: | The Syrian hamster Harderian gland (HG) is an organ that undergoes physiological autophagy in response to oxidative stress induced by porphyrin production. Porphyrin production in the HG has marked sex differences and is closely linked to reproductive function. In the present study, we observed that the estrous cycle and associated estrogen variations may affect oxidative-stress-induced proteolytic processes. In particular, significant changes in autophagic activity were detected during the estrous cycle. Notably, increased activation of macroautophagy as well as chaperone-mediated autophagy in the estrus phase coincided with a minimal antioxidant capability and the highest protein damage levels. By contrast, autophagic machinery was found to be blocked in the diestrus phase, likely due to mammalian target of rapamycin activation, which could be corroborated by the subsequent pS6K activation. Analogous results were observed regarding proteasome activity, which also showed maximal activity in the estrus phase. Interestingly, all these mechanisms were associated with important morphological changes in the HG during the estrous cycle. We observed statistically significant increases in Type II cells, which may be related to extensive autophagy in the estrus phase. Physiologically, this would result in a significant release of porphyrins specifically when females are more receptive. These data support the role of porphyrins as pheromones, as other authors have previously suggested, thus making the HG a scent organ. In addition, these results suggest a porphyrin-based approach to the treatment of porphyria during pregnancy, a condition for which no treatment is currently known. |
| Databáze: | OpenAIRE |
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