Shh signaling through the primary cilium modulates rat oligodendrocyte differentiation
| Autor: | Pablo Lois, Paulina Falcón-Urrutia, Carlos M. Carrasco, Alejandro D. Roth, Verónica Palma |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Receptor
Platelet-Derived Growth Factor alpha animal structures Cyclopamine Cellular differentiation lcsh:Medicine Biology Rats Sprague-Dawley chemistry.chemical_compound Animals Hedgehog Proteins Cilia Sonic hedgehog lcsh:Science Hedgehog Cells Cultured Quinazolinones Platelet-Derived Growth Factor Multidisciplinary lcsh:R Veratrum Alkaloids Oligodendrocyte differentiation Cell Differentiation Hedgehog signaling pathway Cell biology Oligodendroglia stomatognathic diseases nervous system chemistry embryonic structures biology.protein lcsh:Q Signal transduction Platelet-derived growth factor receptor Research Article Signal Transduction |
| Zdroj: | Plos one Artículos CONICYT CONICYT Chile instacron:CONICYT Plos One PLoS ONE PLoS ONE, Vol 10, Iss 7, p e0133567 (2015) |
| Popis: | Primary Cilia (PC) are a very likely place for signal integration where multiple signaling pathways converge. Two major signaling pathways clearly shown to signal through the PC, Sonic Hedgehog (Shh) and PDGF-Rα, are particularly important for the proliferation and differentiation of oligodendrocytes, suggesting that their interaction occurs in or around this organelle. We identified PC in rat oligodendrocyte precursor cells (OPCs) and found that, while easily detectable in early OPCs, PC are lost as these cells progress to terminal differentiation. We confirmed the interaction between these pathways, as cyclopamine inhibition of Hedgehog function impairs both PDGF-mediated OPC proliferation and Shh-dependent cell branching. However, we failed to detect PDGF-Rα localization into the PC. Remarkably, ciliobrevin-mediated disruption of PC and reduction of OPC process extension was counteracted by recombinant Shh treatment, while PDGF had no effect. Therefore, while PDGF-Rα-dependent OPC proliferation and survival most probably does not initiate at the PC, still the integrity of this organelle and cilium-centered pathway is necessary for OPC survival and differentiation. |
| Databáze: | OpenAIRE |
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