Differences in highly pathogenic avian influenza viral pathogenesis and associated early inflammatory response in chickens and ducks
| Autor: | Johanna M.J. Rebel, Lonneke Vervelde, Jacob Post, J.B.W.J. Cornelissen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
animal diseases viruses Viral pathogenesis Interleukin-1beta activated protein-kinase Apoptosis medicine.disease_cause Food Animals Lung innate immune-responses Bacteriologie virus diseases Brain tissue tropism MDA5 Bacteriology Host Pathogen Interaction & Diagnostics Viral Load Immunohistochemistry toll-like receptor-7 medicine.anatomical_structure Ducks Influenza A Virus H7N1 Subtype pekin ducks medicine.symptom h5n1 influenza animal structures cytokine responses Spleen Biology Real-Time Polymerase Chain Reaction Asymptomatic Statistics Nonparametric Species Specificity medicine Animals Poultry Diseases DNA Primers Host Pathogen Interaction & Diagnostics General Immunology and Microbiology Interleukin-6 Macrophages Bacteriology TLR7 Dendritic Cells Virology Host Pathogen Interactie & Diagnostiek Influenza A virus subtype H5N1 Gene Expression Regulation Influenza in Birds Bacteriologie Host Pathogen Interactie & Diagnostiek WIAS Tissue tropism domestic ducks Animal Science and Zoology a virus-infection anas-platyrhynchos Chickens |
| Zdroj: | Avian Pathology 42 (2013) 4 Avian Pathology, 42(4), 347-364 |
| ISSN: | 0307-9457 |
| Popis: | We studied the immunological responses in the lung, brain and spleen of ducks and chickens within the first 7 days after infection with H7N1 highly pathogenic avian influenza (HPAI). Infection with HPAI caused significant morbidity and mortality in chickens, while in ducks the infection was asymptomatic. The HPAI viral mRNA load was higher in all investigated tissues of chickens compared with duck tissues. In the lung, brain and spleen of HPAI-infected chickens, a high, but delayed, pro-inflammatory response of IL-6 and IL-1ß mRNA was induced, including up-regulation of IFN-ß, IFN-¿, TLR3 and MDA-5 mRNA from 1 day post infection (p.i.). Whereas in ducks already at 8 h p.i., a quicker but lower response was found for IL-6, IL-1ß and iNOS mRNA followed by a delayed activation of TLR7, RIG-I, MDA5 and IFN-¿ mRNA response. Virus-infected areas in the lung of chickens co-localized with KUL-01¿ (macrophages, dendritic cells), CD4¿, and CD8a¿ cells, during the first day after infection. However, only KUL-01¿ cells co-localized with the virus after 1 day p.i. In ducks, CVI-ChNL-68.1¿ (macrophage-like cells), CD4¿ and CD8a¿ cells and apoptosis co-localized with the virus within 8 h p.i. Apoptosis was detected in the brain and lung of HPAI-infected chickens after 2 days p.i. and apoptotic cells co-localized with virus-infected areas. In conclusion, excessive delayed cytokine inflammatory responses but inadequate cellular immune responses may contribute to pathogenesis in chickens, while ducks initiate a fast lower cytokine response followed by the activation of major pattern recognition receptors (TLR7, RIG-I, MDA5) and a persistent cellular response. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|