Cloning and Expression in Escherichia coli of the Polysaccharide Depolymerase Associated with Bacteriophage-Infected Erwinia amylovora
| Autor: | Peter A. Vandenbergh, Ronald L. Cole |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Ecology
biology EcoRI Erwinia Molecular cloning biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Enterobacteriaceae PBR322 Microbiology Bacteriophage Restriction map biology.protein medicine Microorganism-Plant Interactions Escherichia coli Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 51:862-864 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.51.4.862-864.1986 |
| Popis: | The bacteriophage-encoded polysaccharide depolymerase produced in Erwinia amylovora has been cloned and expressed in Escherichia coli . The bacteriophage ERA103 genome was observed to consist of five EcoR I fragments, labeled as follows: A, 7.5 kilobases (kb); B, 5.0 kb; C, 2.7 kb; D, 2.1 kb; and E, 1.8 kb. A restriction map for ERA103 was also prepared. Each of the fragments were cloned into the positive-selection vector pOP203(A 2 + ) and pBR322. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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