Expression of VEGF and its receptor genes in intracranial schwannomas

Autor: Tomio Sasaki, Masahiro Mizoguchi, Toshio Uesaka, Akira Nakamizo, Tadahisa Shono, Toru Iwaki, Satoshi O. Suzuki, Hiroaki Niiro
Rok vydání: 2007
Předmět:
Adult
Male
Vascular Endothelial Growth Factor A
Cancer Research
Pathology
medicine.medical_specialty
Angiogenesis
Ubiquitin-Protein Ligases
Astrocytoma
Biology
Immunoenzyme Techniques
chemistry.chemical_compound
Antigens
Neoplasm
medicine
Humans
Cranial Nerve Neoplasms
RNA
Messenger
RNA
Neoplasm
Receptor
Aged
Regulation of gene expression
Neurofibromin 2
Messenger RNA
Vascular Endothelial Growth Factor Receptor-1
Brain Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Topoisomerase
Middle Aged
Vascular Endothelial Growth Factor Receptor-2
DNA-Binding Proteins
Gene Expression Regulation
Neoplastic
Vascular endothelial growth factor
DNA Topoisomerases
Type II
Real-time polymerase chain reaction
Neurology
Oncology
chemistry
biology.protein
Immunohistochemistry
Female
Neurology (clinical)
Neoplasm Recurrence
Local
Glioblastoma
Meningioma
Neurilemmoma
Zdroj: Journal of Neuro-Oncology. 83:259-266
ISSN: 1573-7373
0167-594X
Popis: Vascular endothelial growth factor (VEGF) is considered to be a major regulator of angiogenesis in various brain tumors. In this study, we determined the expression levels of VEGF, and vascular endothelial growth factor receptor (VEGFR)-1 and -2 mRNA in 46 intracranial schwannomas by quantitative real-time PCR, and correlated these with various clinical factors or other molecular markers. We found that these tumors expressed significant amounts of VEGF mRNA in comparison with other brain tumors, including malignant gliomas and meningiomas. In addition, we performed immunohistochemical studies for VEGF and VEGFR-1, and confirmed that these tumors prominently express these proteins. The expression levels of VEGF and VEGFR-1 mRNA in recurrent tumors were higher than those in primary tumors. When we divided patients into two groups according to VEGF mRNA expression in the tumor, there was no significant difference in patient age, gender, or cranial nerves of origin between groups; however, the tumor volume tended to be larger in the high VEGF group than in the low VEGF group. The levels of VEGFR-1 mRNA and neurofibromatosis-2 mRNA in the high VEGF group were significantly greater than those in the low VEGF group. Levels of VEGFR-2 mRNA and DNA topoisomerase IIalpha mRNA, and the MIB-1 labeling index in the high VEGF group were slightly higher than those in the low VEGF group; however, the difference was not statistically significant. Based on these observations, the significance of VEGF and its receptor genes in intracranial schwannomas is discussed.
Databáze: OpenAIRE
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