Expression of VEGF and its receptor genes in intracranial schwannomas
Autor: | Tomio Sasaki, Masahiro Mizoguchi, Toshio Uesaka, Akira Nakamizo, Tadahisa Shono, Toru Iwaki, Satoshi O. Suzuki, Hiroaki Niiro |
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Rok vydání: | 2007 |
Předmět: |
Adult
Male Vascular Endothelial Growth Factor A Cancer Research Pathology medicine.medical_specialty Angiogenesis Ubiquitin-Protein Ligases Astrocytoma Biology Immunoenzyme Techniques chemistry.chemical_compound Antigens Neoplasm medicine Humans Cranial Nerve Neoplasms RNA Messenger RNA Neoplasm Receptor Aged Regulation of gene expression Neurofibromin 2 Messenger RNA Vascular Endothelial Growth Factor Receptor-1 Brain Neoplasms Reverse Transcriptase Polymerase Chain Reaction Topoisomerase Middle Aged Vascular Endothelial Growth Factor Receptor-2 DNA-Binding Proteins Gene Expression Regulation Neoplastic Vascular endothelial growth factor DNA Topoisomerases Type II Real-time polymerase chain reaction Neurology Oncology chemistry biology.protein Immunohistochemistry Female Neurology (clinical) Neoplasm Recurrence Local Glioblastoma Meningioma Neurilemmoma |
Zdroj: | Journal of Neuro-Oncology. 83:259-266 |
ISSN: | 1573-7373 0167-594X |
Popis: | Vascular endothelial growth factor (VEGF) is considered to be a major regulator of angiogenesis in various brain tumors. In this study, we determined the expression levels of VEGF, and vascular endothelial growth factor receptor (VEGFR)-1 and -2 mRNA in 46 intracranial schwannomas by quantitative real-time PCR, and correlated these with various clinical factors or other molecular markers. We found that these tumors expressed significant amounts of VEGF mRNA in comparison with other brain tumors, including malignant gliomas and meningiomas. In addition, we performed immunohistochemical studies for VEGF and VEGFR-1, and confirmed that these tumors prominently express these proteins. The expression levels of VEGF and VEGFR-1 mRNA in recurrent tumors were higher than those in primary tumors. When we divided patients into two groups according to VEGF mRNA expression in the tumor, there was no significant difference in patient age, gender, or cranial nerves of origin between groups; however, the tumor volume tended to be larger in the high VEGF group than in the low VEGF group. The levels of VEGFR-1 mRNA and neurofibromatosis-2 mRNA in the high VEGF group were significantly greater than those in the low VEGF group. Levels of VEGFR-2 mRNA and DNA topoisomerase IIalpha mRNA, and the MIB-1 labeling index in the high VEGF group were slightly higher than those in the low VEGF group; however, the difference was not statistically significant. Based on these observations, the significance of VEGF and its receptor genes in intracranial schwannomas is discussed. |
Databáze: | OpenAIRE |
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